8TWE

Cryo-EM structure of the PP2A:B55-FAM122A complex, B55 body

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform	polymer	584	64958.0	1	UniProt (P30153) Pfam (PF02985) In PDB	Homo sapiens (human)	Medium tumor antigen-associated 61 kDa protein,PP2A subunit A isoform PR65-alpha,PP2A subunit A isoform R1-alpha
2	B	Serine/threonine-protein phosphatase 2A 55 kDa regulatory subunit B alpha isoform	polymer	451	52101.3	1	UniProt (P63151) In PDB	Homo sapiens (human)	PP2A subunit B isoform B55-alpha,PP2A subunit B isoform PR55-alpha,PP2A subunit B isoform R2-alpha,PP2A subunit B isoform alpha
3	C	Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform	polymer	311	35845.4	1	UniProt (P67775) Pfam (PF00149) In PDB	Homo sapiens (human)	PP2A-alpha,Replication protein C,RP-C
4	D	PPP2R1A-PPP2R2A-interacting phosphatase regulator 1	polymer	95	10680.9	1	UniProt (Q96E09) In PDB	Homo sapiens (human)	PABIR family member 1

Sequence modifications

A: 9 - 589 (UniProt: P30153)

PDB	External Database	Details
Gly 6	-	expression tag
His 7	-	expression tag
Met 8	-	expression tag

B: 2 - 447 (UniProt: P63151)

PDB	External Database	Details
Gly -3	-	expression tag
His -2	-	expression tag
Met -1	-	expression tag
Gly 0	-	expression tag
Ser 1	-	expression tag

C: 1 - 309 (UniProt: P67775)

PDB	External Database	Details
Gly -1	-	expression tag
His 0	-	expression tag

D: 29 - 120 (UniProt: Q96E09)

PDB	External Database	Details
Gly 26	-	expression tag
His 27	-	expression tag
Met 28	-	expression tag
Ser 47	Thr 47	conflict
Asp 53	Ala 53	conflict
Arg 62	Ser 62	conflict

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	163585.6
All*	Total formula weight	163585.6

*Water molecules are not included.