8TAZ

Cryo-EM structure of mink variant Y453F trimeric spike protein bound to one mink ACE2 receptors

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C	Spike glycoprotein	polymer	1269	140244.1	3	UniProt (P0DTC2) Pfam (PF16451) Pfam (PF09408) Pfam (PF19209) Pfam (PF01601) In PDB	Severe acute respiratory syndrome coronavirus 2	S glycoprotein,E2,Peplomer protein
2	D	Angiotensin-converting enzyme	polymer	771	89375.5	1	UniProt (A0A7T0Q2W2) Pfam (PF01401) Pfam (PF16959) In PDB	Neogale vison (American mink)

Sequence modifications

A, B, C: 1 - 1208 (UniProt: P0DTC2)

PDB	External Database	Details
-	His 69	deletion
-	Val 70	deletion
Phe 453	Tyr 453	variant
Gly 614	Asp 614	engineered mutation
Gly 682	Arg 682	engineered mutation
Ser 683	Arg 683	engineered mutation
Ser 685	Arg 685	engineered mutation
Pro 817	Phe 817	engineered mutation
Pro 892	Ala 892	engineered mutation
Pro 899	Ala 899	engineered mutation
Pro 942	Ala 942	engineered mutation
Pro 986	Lys 986	engineered mutation
Pro 987	Val 987	engineered mutation
Gly 1209	-	expression tag
Ser 1210	-	expression tag
Gly 1211	-	expression tag
Ser 1212	-	expression tag
Gly 1213	-	expression tag
Ser 1214	-	expression tag
Gly 1215	-	expression tag
Ser 1216	-	expression tag
Gly 1217	-	expression tag
Tyr 1218	-	expression tag
Ile 1219	-	expression tag
Pro 1220	-	expression tag
Glu 1221	-	expression tag
Ala 1222	-	expression tag
Pro 1223	-	expression tag
Arg 1224	-	expression tag
Asp 1225	-	expression tag
Gly 1226	-	expression tag
Gln 1227	-	expression tag
Ala 1228	-	expression tag
Tyr 1229	-	expression tag
Val 1230	-	expression tag
Arg 1231	-	expression tag
Lys 1232	-	expression tag
Asp 1233	-	expression tag
Gly 1234	-	expression tag
Glu 1235	-	expression tag
Trp 1236	-	expression tag
Val 1237	-	expression tag
Leu 1238	-	expression tag
Leu 1239	-	expression tag
Ser 1240	-	expression tag
Thr 1241	-	expression tag
Phe 1242	-	expression tag
Leu 1243	-	expression tag
Gly 1244	-	expression tag
Ser 1245	-	expression tag
Gly 1246	-	expression tag
Ser 1247	-	expression tag
Gly 1248	-	expression tag
Ser 1249	-	expression tag
Gly 1250	-	expression tag
His 1251	-	expression tag
His 1252	-	expression tag
His 1253	-	expression tag
His 1254	-	expression tag
His 1255	-	expression tag
His 1256	-	expression tag
Gly 1257	-	expression tag
Leu 1258	-	expression tag
Asn 1259	-	expression tag
Asp 1260	-	expression tag
Ile 1261	-	expression tag
Phe 1262	-	expression tag
Glu 1263	-	expression tag
Ala 1264	-	expression tag
Gln 1265	-	expression tag
Lys 1266	-	expression tag
Ile 1267	-	expression tag
Glu 1268	-	expression tag
Trp 1269	-	expression tag
His 1270	-	expression tag
Glu 1271	-	expression tag

D: 1 - 739 (UniProt: A0A7T0Q2W2)

PDB	External Database	Details
Gly 740	-	expression tag
Ser 741	-	expression tag
Gly 742	-	expression tag
Ser 743	-	expression tag
Gly 744	-	expression tag
Ser 745	-	expression tag
Gly 746	-	expression tag
His 747	-	expression tag
His 748	-	expression tag
His 749	-	expression tag
His 750	-	expression tag
His 751	-	expression tag
His 752	-	expression tag
Gly 753	-	expression tag
Ser 754	-	expression tag
Gly 755	-	expression tag
Ser 756	-	expression tag
Gly 757	-	expression tag
Leu 758	-	expression tag
Asn 759	-	expression tag
Asp 760	-	expression tag
Ile 761	-	expression tag
Phe 762	-	expression tag
Glu 763	-	expression tag
Ala 764	-	expression tag
Gln 765	-	expression tag
Lys 766	-	expression tag
Ile 767	-	expression tag
Glu 768	-	expression tag
Trp 769	-	expression tag
His 770	-	expression tag
Glu 771	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	510107.8
All*	Total formula weight	510107.8

*Water molecules are not included.