8SG4

E1435Q Ycf1 mutant in dephosphorylated state

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Metal resistance protein YCF1	polymer	1560	176209.6	1	UniProt (P39109) Pfam (PF00664) Pfam (PF00005) In PDB	Saccharomyces cerevisiae (brewer's yeast)	ABC-type Cd(2+) transporter,ABC-type glutathione-S-conjugate transporter,Yeast cadmium factor 1

Sequence modifications

A: 1 - 1515 (UniProt: P39109)

PDB	External Database	Details
Met -23	-	initiating methionine
Ala -22	-	expression tag
Ser -21	-	expression tag
Asp -20	-	expression tag
Tyr -19	-	expression tag
Lys -18	-	expression tag
Asp -17	-	expression tag
Asp -16	-	expression tag
Asp -15	-	expression tag
Asp -14	-	expression tag
Lys -13	-	expression tag
Gly -12	-	expression tag
Ala -11	-	expression tag
Leu -10	-	expression tag
Glu -9	-	expression tag
Val -8	-	expression tag
Leu -7	-	expression tag
Phe -6	-	expression tag
Gln -5	-	expression tag
Gly -4	-	expression tag
Pro -3	-	expression tag
Ser -2	-	expression tag
Ser -1	-	expression tag
Pro 0	-	expression tag
Gln 1435	Glu 1435	engineered mutation
Gly 1516	-	expression tag
Leu 1517	-	expression tag
Val 1518	-	expression tag
Pro 1519	-	expression tag
Arg 1520	-	expression tag
Gly 1521	-	expression tag
Ser 1522	-	expression tag
Ser 1523	-	expression tag
Ala 1524	-	expression tag
His 1525	-	expression tag
His 1526	-	expression tag
His 1527	-	expression tag
His 1528	-	expression tag
His 1529	-	expression tag
His 1530	-	expression tag
His 1531	-	expression tag
His 1532	-	expression tag
His 1533	-	expression tag
His 1534	-	expression tag
Gly 1535	-	expression tag
Ala 1536	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	176209.6
All*	Total formula weight	176209.6

*Water molecules are not included.