8QU2

NF-YB/C Heterodimer in Complex with a 16-mer NF-YA-derived Peptide Stabilized with C8-Hydrocarbon Linker

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Nuclear transcription factor Y subunit alpha	polymer	18	2061.6	1	UniProt (P23511) In PDB	Homo sapiens	CAAT box DNA-binding protein subunit A,Nuclear transcription factor Y subunit A,NF-YA
2	B	Nuclear transcription factor Y subunit beta	polymer	95	10876.5	1	UniProt (P25208) Pfam (PF00808) In PDB	Homo sapiens (human)	CAAT box DNA-binding protein subunit B,Nuclear transcription factor Y subunit B,NF-YB
3	C	Nuclear transcription factor Y subunit gamma	polymer	96	11258.2	1	UniProt (Q13952) Pfam (PF00125) In PDB	Homo sapiens (human)	CAAT box DNA-binding protein subunit C,Nuclear transcription factor Y subunit C,NF-YC,Transactivator HSM-1/2
4	A	2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL	non-polymer		122.1	1	Chemie (TRS)
5	A, B	FORMIC ACID	non-polymer		46.0	2	Chemie (FMT)
6	C	1,2-ETHANEDIOL	non-polymer		62.1	2	Chemie (EDO)
7		water	water		18.0	139	Chemie (HOH)

Sequence modifications

A: 270 - 285 (UniProt: P23511)

PDB	External Database	Details
Ace 269	-	acetylation
Nle 272	Tyr 272	engineered mutation
Nh2 286	-	amidation

B: 51 - 143 (UniProt: P25208)

PDB	External Database	Details
Gly 49	-	expression tag
Pro 50	-	expression tag

C: 27 - 120 (UniProt: Q13952)

PDB	External Database	Details
Gly 25	-	expression tag
Pro 26	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	24196.3
Non-Polymers*	Number of molecules	5
	Total formula weight	338.3
All*	Total formula weight	24534.6

*Water molecules are not included.