8QCA

CryoEM structure of a S. Cerevisiae Ski2387 complex in the closed state bound to RNA

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Antiviral helicase SKI2	polymer	1287	146259.1	1	UniProt (P35207) Pfam (PF17911) Pfam (PF00270) Pfam (PF00271) Pfam (PF21408) Pfam (PF21409) Pfam (PF08148) In PDB	Saccharomyces cerevisiae (baker's yeast)	Superkiller protein 2
2	B	Superkiller protein 3	polymer	1436	164278.5	1	UniProt (P17883) Pfam (PF18833) In PDB	Saccharomyces cerevisiae (baker's yeast)
3	C, D	Antiviral protein SKI8	polymer	397	44283.5	2	UniProt (Q02793) Pfam (PF00400) In PDB	Saccharomyces cerevisiae (baker's yeast)
4	E	Superkiller protein 7	polymer	250	28345.1	1	UniProt (Q08491) In PDB	Saccharomyces cerevisiae (baker's yeast)
5	X	RNA (5'-R(P*UP*UP*UP*U)-3')	polymer	30	9140.0	1		Saccharomyces cerevisiae

Sequence modifications

B: 1 - 1432 (UniProt: P17883)

PDB	External Database	Details
Gly -3	-	expression tag
Pro -2	-	expression tag
Asp -1	-	expression tag
Ser 0	-	expression tag

E: 1 - 235 (UniProt: Q08491)

PDB	External Database	Details
Gly -3	-	expression tag
Pro -2	-	expression tag
Asp -1	-	expression tag
Ser 0	-	expression tag
Ala 236	-	expression tag
Gly 237	-	expression tag
Ser 238	-	expression tag
Trp 239	-	expression tag
Ser 240	-	expression tag
His 241	-	expression tag
Pro 242	-	expression tag
Gln 243	-	expression tag
Phe 244	-	expression tag
Glu 245	-	expression tag
Lys 246	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	436589.8
All*	Total formula weight	436589.8

*Water molecules are not included.