8J8F

Monkeypox virus DNA replication holoenzyme F8, A22 and E4 in complex with a DNA duplex and dCTP

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	DNA polymerase	polymer	1029	120041.2	1	UniProt (Q5IXW8) Pfam (PF08452) Pfam (PF03104) Pfam (PF08408) Pfam (PF00136) In PDB	Monkeypox virus
2	B	E4R	polymer	241	27883.7	1	UniProt (Q5IXS4) In PDB	Monkeypox virus
3	C	DNA polymerase processivity factor component A20	polymer	426	49203.9	1	UniProt (Q5IXP2) Pfam (PF05941) In PDB	Monkeypox virus
4	P	DNA (5'-D(P*AP*GP*CP*TP*GP*CP*TP*AP*TP*GP*AP*GP*AP*TP*TP*AP*AP*GP*TP*TP*AP*T)-3')	polymer	24	7438.8	1		DNA molecule
5	T	DNA (5'-D(P*GP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*GP*AP*TP*AP*AP*CP*TP*TP*AP*AP*TP*CP*TP*CP*AP*CP*AP*TP*AP*GP*CP*AP*GP*CP*T)-3')	polymer	38	11605.5	1		DNA molecule
6	A	2'-DEOXYCYTIDINE-5'-TRIPHOSPHATE	non-polymer		467.2	1	Chemie (DCP)
7	A	CALCIUM ION	non-polymer		40.1	3	Chemie (CA)

Sequence modifications

A: 1 - 1006 (UniProt: Q5IXW8)

PDB	External Database	Details
Met -22	-	initiating methionine
His -21	-	expression tag
His -20	-	expression tag
His -19	-	expression tag
His -18	-	expression tag
His -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
Asp -13	-	expression tag
Tyr -12	-	expression tag
Asp -11	-	expression tag
Ile -10	-	expression tag
Pro -9	-	expression tag
Thr -8	-	expression tag
Thr -7	-	expression tag
Glu -6	-	expression tag
Asn -5	-	expression tag
Leu -4	-	expression tag
Tyr -3	-	expression tag
Phe -2	-	expression tag
Gln -1	-	expression tag
Gly 0	-	expression tag
Phe 108	Leu 108	engineered mutation
Leu 411	Trp 411	engineered mutation

B: 1 - 218 (UniProt: Q5IXS4)

PDB	External Database	Details
Met -22	-	initiating methionine
His -21	-	expression tag
His -20	-	expression tag
His -19	-	expression tag
His -18	-	expression tag
His -17	-	expression tag
His -16	-	expression tag
Asp -15	-	expression tag
Tyr -14	-	expression tag
Asp -13	-	expression tag
Ile -12	-	expression tag
Pro -11	-	expression tag
Thr -10	-	expression tag
Thr -9	-	expression tag
Glu -8	-	expression tag
Asn -7	-	expression tag
Leu -6	-	expression tag
Tyr -5	-	expression tag
Phe -4	-	expression tag
Gln -3	-	expression tag
Gly -2	-	expression tag
Ala -1	-	expression tag
Ser 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	216173.1
Non-Polymers*	Number of molecules	4
	Total formula weight	587.4
All*	Total formula weight	216760.5

*Water molecules are not included.