8J6J

Cryo-EM structure of thehydroxycarboxylic acid receptor 2-Gi protein complex bound with GSK256073

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Guanine nucleotide-binding protein G(i) subunit alpha-1	polymer	354	40414.0	1	UniProt (P63096) Pfam (PF00503) In PDB	Homo sapiens (human)	Adenylate cyclase-inhibiting G alpha protein
2	B	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	370	40095.8	1	UniProt (P62873) Pfam (PF00400) In PDB	Homo sapiens (human)
3	G	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	70	7729.9	1	UniProt (P59768) Pfam (PF00631) In PDB	Homo sapiens (human)	G gamma-I
4	H	Scfv16	polymer	247	26277.3	1		Homo sapiens
5	R	Hydroxycarboxylic acid receptor 2,hydroxycarboxylic acid receptor 2	polymer	488	56107.6	1	UniProt (Q8TDS4) Pfam (PF00001) In PDB	Homo sapiens (human)	G-protein coupled receptor 109A,G-protein coupled receptor HM74A,Niacin receptor 1,Nicotinic acid receptor
6	R	8-chloranyl-3-pentyl-7H-purine-2,6-dione	non-polymer		256.7	1	Chemie (OKL)

Sequence modifications

A: 1 - 354 (UniProt: P63096)

PDB	External Database	Details
Asn 47	Ser 47	engineered mutation
Ala 203	Gly 203	engineered mutation
Ala 245	Glu 245	engineered mutation
Ser 326	Ala 326	engineered mutation

B: 2 - 340 (UniProt: P62873)

PDB	External Database	Details
Gly -3	-	expression tag
Ser -2	-	expression tag
Leu -1	-	expression tag
Leu 0	-	expression tag
Gln 1	-	expression tag
Gly 341	-	expression tag
Ser 342	-	expression tag
Ser 343	-	expression tag
Gly 344	-	expression tag
Gly 345	-	expression tag
Gly 346	-	expression tag
Gly 347	-	expression tag
Ser 348	-	expression tag
Gly 349	-	expression tag
Gly 350	-	expression tag
Gly 351	-	expression tag
Gly 352	-	expression tag
Ser 353	-	expression tag
Ser 354	-	expression tag
Gly 355	-	expression tag
Val 356	-	expression tag
Ser 357	-	expression tag
Gly 358	-	expression tag
Trp 359	-	expression tag
Arg 360	-	expression tag
Leu 361	-	expression tag
Phe 362	-	expression tag
Lys 363	-	expression tag
Lys 364	-	expression tag
Ile 365	-	expression tag
Ser 366	-	expression tag

R: 1 - 330 (UniProt: Q8TDS4)
R: 331 - 488 (PDB: 8J6J)

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	170624.7
Non-Polymers*	Number of molecules	1
	Total formula weight	256.7
All*	Total formula weight	170881.4

*Water molecules are not included.