8IHX

X-ray crystal structure of N372D mutant of endo-1,4-beta glucanase from Eisenia fetida

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Endoglucanase	polymer	462	51706.1	1	UniProt (I2FI81) Pfam (PF00759) In PDB	Eisenia fetida (common brandling worm)
2	A	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)
3	A	CALCIUM ION	non-polymer		40.1	1	Chemie (CA)
4	A	SODIUM ION	non-polymer		23.0	1	Chemie (NA)
5	A	GLYCEROL	non-polymer		92.1	1	Chemie (GOL)
6		water	water		18.0	421	Chemie (HOH)

Sequence modifications

A: 22 - 456 (UniProt: I2FI81)

PDB	External Database	Details
Glu 18	-	expression tag
Ala 19	-	expression tag
Glu 20	-	expression tag
Phe 21	-	expression tag
Asp 372	Asn 372	engineered mutation
Phe 457	-	expression tag
Leu 458	-	expression tag
Glu 459	-	expression tag
Gln 460	-	expression tag
Lys 461	-	expression tag
Leu 462	-	expression tag
Ile 463	-	expression tag
Ser 464	-	expression tag
Glu 465	-	expression tag
Glu 466	-	expression tag
Asp 467	-	expression tag
Leu 468	-	expression tag
Asn 469	-	expression tag
Ser 470	-	expression tag
Ala 471	-	expression tag
Val 472	-	expression tag
Asp 473	-	expression tag
His 474	-	expression tag
His 475	-	expression tag
His 476	-	expression tag
His 477	-	expression tag
His 478	-	expression tag
His 479	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	51706.1
Non-Polymers*	Number of molecules	4
	Total formula weight	179.5
All*	Total formula weight	51885.5

*Water molecules are not included.