8I9S

Structure of Apo-C3aR-Go complex (Titan)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	C	C3a anaphylatoxin chemotactic receptor	polymer	538	59808.4	1	UniProt (Q16581) Pfam (PF00001) In PDB	Homo sapiens (human)	C3AR,C3a-R
2	A	Guanine nucleotide-binding protein G(o) subunit alpha	polymer	250	28193.9	1	UniProt (P09471) Pfam (PF00503) In PDB	Homo sapiens (human)
3	B	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	350	38534.1	1	UniProt (P62873) Pfam (PF00400) In PDB	Homo sapiens (human)	Transducin beta chain 1
4	G	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7861.1	1	UniProt (P59768) Pfam (PF00631) In PDB	Homo sapiens (human)	G gamma-I
5	H	Antibody fragment - ScFv16	polymer	248	26466.5	1		Mus musculus

Sequence modifications

C: 2 - 482 (UniProt: Q16581)

PDB	External Database	Details
Met -55	-	initiating methionine
Gly -54	-	expression tag
Lys -53	-	expression tag
Thr -52	-	expression tag
Ile -51	-	expression tag
Ile -50	-	expression tag
Ala -49	-	expression tag
Leu -48	-	expression tag
Ser -47	-	expression tag
Tyr -46	-	expression tag
Ile -45	-	expression tag
Phe -44	-	expression tag
Cys -43	-	expression tag
Leu -42	-	expression tag
Val -41	-	expression tag
Phe -40	-	expression tag
Ala -39	-	expression tag
Asp -38	-	expression tag
Tyr -37	-	expression tag
Lys -36	-	expression tag
Asp -35	-	expression tag
Asp -34	-	expression tag
Asp -33	-	expression tag
Asp -32	-	expression tag
Ala -31	-	expression tag
Ala -30	-	expression tag
Asn -29	-	expression tag
Phe -28	-	expression tag
Thr -27	-	expression tag
Pro -26	-	expression tag
Val -25	-	expression tag
Asn -24	-	expression tag
Gly -23	-	expression tag
Ser -22	-	expression tag
Ser -21	-	expression tag
Gly -20	-	expression tag
Asn -19	-	expression tag
Gln -18	-	expression tag
Ser -17	-	expression tag
Val -16	-	expression tag
Arg -15	-	expression tag
Leu -14	-	expression tag
Val -13	-	expression tag
Thr -12	-	expression tag
Ser -11	-	expression tag
Ser -10	-	expression tag
Ser -9	-	expression tag
Leu -8	-	expression tag
Glu -7	-	expression tag
Val -6	-	expression tag
Leu -5	-	expression tag
Phe -4	-	expression tag
Gln -3	-	expression tag
Gly -2	-	expression tag
Pro -1	-	expression tag
Gly 0	-	expression tag
Ser 1	-	expression tag

A: 4 - 173 (UniProt: P09471)

PDB	External Database	Details
Met -11	-	initiating methionine
Gly -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
Glu -3	-	expression tag
Asn -2	-	expression tag
Leu -1	-	expression tag
Tyr 0	-	expression tag
Phe 1	-	expression tag
Gln 2	-	expression tag
Gly 3	-	expression tag
Asp 42	Gly 42	engineered mutation
Asn 43	Glu 43	engineered mutation

A: 182 - 354 (UniProt: P09471)

PDB	External Database	Details
Gly 174	-	linker
Gly 175	-	linker
Ser 176	-	linker
Gly 177	-	linker
Gly 178	-	linker
Ser 179	-	linker
Gly 180	-	linker
Gly 181	-	linker
Asp 227	Ala 227	engineered mutation
Asp 230	Gly 230	engineered mutation
Ala 332	Ile 332	engineered mutation
Ile 335	Val 335	engineered mutation

B: 2 - 340 (UniProt: P62873)

PDB	External Database	Details
Met -9	-	initiating methionine
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
Ser 0	-	expression tag
Gly 1	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	160864.1
All*	Total formula weight	160864.1

*Water molecules are not included.