8HA0

Molecular recognition of two endogenous hormones by the human parathyroid hormone receptor-1

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Guanine nucleotide-binding protein g(s) subunit alpha	polymer	361	41879.5	1		Bos taurus (cattle)
2	B	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	400	43706.8	1	UniProt (P54311) Pfam (PF00400) In PDB	Rattus norvegicus (rat)	Transducin beta chain 1
3	G	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7861.1	1	UniProt (P63212) Pfam (PF00631) In PDB	Bos taurus (cattle)	G gamma-I
4	N	Nanobody 35	polymer	140	15343.0	1		synthetic construct
5	P	Parathyroid hormone	polymer	34	4125.8	1	UniProt (P01270) Pfam (PF01279) In PDB	Homo sapiens (human)	PTH,Parathormone,Parathyrin
6	R	Parathyroid hormone/parathyroid hormone-related peptide receptor	polymer	476	54506.7	1	UniProt (Q03431) Pfam (PF02793) Pfam (PF00002) In PDB	Homo sapiens (human)	PTH/PTHrP type I receptor,PTH/PTHr receptor,Parathyroid hormone 1 receptor,PTH1 receptor
7	R	CHOLESTEROL	non-polymer		386.7	4	Chemie (CLR)
8	R	PALMITIC ACID	non-polymer		256.4	3	Chemie (PLM)

Sequence modifications

B: 2 - 340 (UniProt: P54311)

PDB	External Database	Details
Met -33	-	expression tag
His -32	-	expression tag
His -31	-	expression tag
His -30	-	expression tag
His -29	-	expression tag
His -28	-	expression tag
His -27	-	expression tag
Ser -26	-	expression tag
Ser -25	-	expression tag
Gly -24	-	expression tag
Leu -23	-	expression tag
Val -22	-	expression tag
Pro -21	-	expression tag
Arg -20	-	expression tag
Gly -19	-	expression tag
Ser -18	-	expression tag
His -17	-	expression tag
Met -16	-	expression tag
Ala -15	-	expression tag
Ser -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
Gly -3	-	expression tag
Ser -2	-	expression tag
Leu -1	-	expression tag
Leu 0	-	expression tag
Gln 1	-	expression tag
Gly 341	-	expression tag
Ser 342	-	expression tag
Ser 343	-	expression tag
Gly 344	-	expression tag
Gly 345	-	expression tag
Gly 346	-	expression tag
Gly 347	-	expression tag
Ser 348	-	expression tag
Gly 349	-	expression tag
Gly 350	-	expression tag
Gly 351	-	expression tag
Gly 352	-	expression tag
Ser 353	-	expression tag
Ser 354	-	expression tag
Gly 355	-	expression tag
Val 356	-	expression tag
Ser 357	-	expression tag
Gly 358	-	expression tag
Trp 359	-	expression tag
Arg 360	-	expression tag
Leu 361	-	expression tag
Phe 362	-	expression tag
Lys 363	-	expression tag
Lys 364	-	expression tag
Ile 365	-	expression tag
Ser 366	-	expression tag

R: 27 - 502 (UniProt: Q03431)

PDB	External Database	Details
Ala 188	Gly 188	conflict
Arg 484	Lys 484	conflict

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	167422.9
Non-Polymers*	Number of molecules	7
	Total formula weight	2315.9
All*	Total formula weight	169738.8

*Water molecules are not included.