8EIT

Structure of FFAR1-Gq complex bound to DHA

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	A modified Guanine nucleotide-binding protein G(q) subunit alpha	polymer	238	27638.5	1		Homo sapiens (human)
2	B	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	340	37416.9	1	UniProt (P62873) Pfam (PF00400) In PDB	Homo sapiens (human)	Transducin beta chain 1
3	C	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	82	9137.5	1	UniProt (P59768) Pfam (PF00631) In PDB	Homo sapiens (human)	G gamma-I
4	E	scFv16	polymer	318	34930.9	1		Homo sapiens (Human)
5	R	Free fatty acid receptor 1	polymer	501	54298.8	1	UniProt (P00720) UniProt (O14842) Pfam (PF00001) Pfam (PF00959) In PDB	Tequatrovirus T4	G-protein coupled receptor 40
6	R	DOCOSA-4,7,10,13,16,19-HEXAENOIC ACID	non-polymer		328.5	1	Chemie (HXA)

Sequence modifications

C: 2 - 71 (UniProt: P59768)

PDB	External Database	Details
Met -10	-	expression tag
Gly -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
Gly 0	-	expression tag
Gly 1	-	expression tag

R: -168 - -9 (UniProt: P00720)

PDB	External Database	Details
Met -200	-	initiating methionine
Gly -199	-	expression tag
Lys -198	-	expression tag
Thr -197	-	expression tag
Ile -196	-	expression tag
Ile -195	-	expression tag
Ala -194	-	expression tag
Leu -193	-	expression tag
Ser -192	-	expression tag
Tyr -191	-	expression tag
Ile -190	-	expression tag
Phe -189	-	expression tag
Cys -188	-	expression tag
Leu -187	-	expression tag
Val -186	-	expression tag
Phe -185	-	expression tag
Ala -184	-	expression tag
Asp -183	-	expression tag
Tyr -182	-	expression tag
Lys -181	-	expression tag
Asp -180	-	expression tag
Asp -179	-	expression tag
Asp -178	-	expression tag
Asp -177	-	expression tag
Ala -176	-	expression tag
Glu -175	-	expression tag
Asn -174	-	expression tag
Leu -173	-	expression tag
Tyr -172	-	expression tag
Phe -171	-	expression tag
Gln -170	-	expression tag
Gly -169	-	expression tag
Gly -158	Arg 12	conflict
Thr -116	Cys 54	conflict
Ala -73	Cys 97	conflict
Arg -33	Ile 137	conflict
Leu -8	-	linker
Glu -7	-	linker
Val -6	-	linker
Leu -5	-	linker
Phe -4	-	linker
Gln -3	-	linker
Gly -2	-	linker
Pro -1	-	linker
Glu 0	-	linker
Phe 1	-	linker

R: 2 - 300 (UniProt: O14842)

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	163422.5
Non-Polymers*	Number of molecules	1
	Total formula weight	328.5
All*	Total formula weight	163751.0

*Water molecules are not included.