8E4Q

The closed C0-state flycatcher TRPM8 structure in complex with PI(4,5)P2

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, D, C	Transient receptor potential cation channel subfamily M member 8	polymer	1135	131343.4	4	UniProt (U3JD03) Pfam (PF18139) Pfam (PF00520) UniProt (by SIFTS) (A0A803VFM3) In PDB	Ficedula albicollis (Collared flycatcher)
2	A, B, D, C	[(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate	non-polymer		746.6	4	Chemie (PIO)

Sequence modifications

A, B, D, C: 1 - 1075 (UniProt: U3JD03)

PDB	External Database	Details
Met -1	-	expression tag
Ala 0	-	expression tag
Ala 535	Phe 558	engineered mutation
Asp 538	Tyr 561	engineered mutation
Asp 539	Tyr 562	engineered mutation
Glu 1076	-	expression tag
Met 1077	-	expression tag
Val 1078	-	expression tag
His 1079	-	expression tag
Arg 1080	-	expression tag
Phe 1081	-	expression tag
Arg 1082	-	expression tag
Gln 1083	-	expression tag
Leu 1084	-	expression tag
Asp 1085	-	expression tag
Ala 1086	-	expression tag
Lys 1087	-	expression tag
Leu 1088	-	expression tag
Ser 1089	-	expression tag
Asp 1090	-	expression tag
Leu 1091	-	expression tag
Lys 1092	-	expression tag
Gly 1093	-	expression tag
Leu 1094	-	expression tag
Leu 1095	-	expression tag
Lys 1096	-	expression tag
Glu 1097	-	expression tag
Ile 1098	-	expression tag
Ser 1099	-	expression tag
Ser 1100	-	expression tag
Lys 1101	-	expression tag
Ile 1102	-	expression tag
Lys 1103	-	expression tag
Ser 1104	-	expression tag
Asn 1105	-	expression tag
Ser 1106	-	expression tag
Leu 1107	-	expression tag
Glu 1108	-	expression tag
Val 1109	-	expression tag
Leu 1110	-	expression tag
Phe 1111	-	expression tag
Gln 1112	-	expression tag
Gly 1113	-	expression tag
Pro 1114	-	expression tag
Asp 1115	-	expression tag
Tyr 1116	-	expression tag
Lys 1117	-	expression tag
Asp 1118	-	expression tag
Asp 1119	-	expression tag
Asp 1120	-	expression tag
Asp 1121	-	expression tag
Lys 1122	-	expression tag
Ala 1123	-	expression tag
His 1124	-	expression tag
His 1125	-	expression tag
His 1126	-	expression tag
His 1127	-	expression tag
His 1128	-	expression tag
His 1129	-	expression tag
His 1130	-	expression tag
His 1131	-	expression tag
His 1132	-	expression tag
His 1133	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	525373.6
Non-Polymers*	Number of molecules	4
	Total formula weight	2986.3
All*	Total formula weight	528359.9

*Water molecules are not included.