8CO5

The surface-engineered photosensory module (PAS-GAF-PHY) of the bacterial phytochrome Agp1 (AtBphP1) in the Pr form with parallel dimer formation

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Bacteriophytochrome protein	polymer	503	54736.9	1	UniProt (Q7CY45) Pfam (PF08446) Pfam (PF01590) Pfam (PF00360) In PDB	Agrobacterium fabrum str. C58
2	A	3-[5-[(4-ethenyl-3-methyl-5-oxidanylidene-pyrrol-2-yl)methyl]-2-[[5-[(3-ethyl-4-methyl-5-oxidanylidene-pyrrol-2-yl)methyl]-3-(3-hydroxy-3-oxopropyl)-4-methyl-1~{H}-pyrrol-2-yl]methyl]-4-methyl-1~{H}-pyrrol-3-yl]propanoic acid	non-polymer		586.7	1	Chemie (V8U)
3	A	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)
4		water	water		18.0	83	Chemie (HOH)

Sequence modifications

A: 10 - 504 (UniProt: Q7CY45)

PDB	External Database	Details
Ala 86	Glu 77	engineered mutation
Ala 87	Glu 78	engineered mutation
Ala 336	Glu 327	engineered mutation
Ala 337	Lys 328	engineered mutation
Leu 505	-	expression tag
Glu 506	-	expression tag
His 507	-	expression tag
His 508	-	expression tag
His 509	-	expression tag
His 510	-	expression tag
His 511	-	expression tag
His 512	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	54736.9
Non-Polymers*	Number of molecules	2
	Total formula weight	611.0
All*	Total formula weight	55347.9

*Water molecules are not included.