8BEH

Cryo-EM structure of the Arabidopsis thaliana I+III2 supercomplex (CI membrane tip)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	L	NADH-ubiquinone oxidoreductase chain 5	polymer	669	74498.0	1	UniProt (P29388) Pfam (PF00662) Pfam (PF00361) Pfam (PF06455) In PDB	Arabidopsis thaliana (thale cress)	NADH dehydrogenase subunit 5
2	M	NADH-ubiquinone oxidoreductase chain 4	polymer	495	55995.7	1	UniProt (P93313) Pfam (PF00361) In PDB	Arabidopsis thaliana (thale cress)	NADH dehydrogenase subunit 4
3	T	Acyl carrier protein 1, mitochondrial	polymer	122	13735.6	1	UniProt (P53665) Pfam (PF00550) In PDB	Arabidopsis thaliana (thale cress)	MtACP-1,ACP,NADH-ubiquinone oxidoreductase 9.6 kDa subunit
4	c	Transmembrane protein	polymer	88	9876.4	1	UniProt (Q8VZT9) In PDB	Arabidopsis thaliana (thale cress)
5	g	ESSS subunit of NADH:ubiquinone oxidoreductase (Complex I) protein	polymer	114	12648.3	1	UniProt (Q9SLC8) In PDB	Arabidopsis thaliana (thale cress)
6	i	At1g67350	polymer	98	11808.2	1	UniProt (Q9FYF8) Pfam (PF17250) In PDB	Arabidopsis thaliana (thale cress)	F1N21.17,NADH-ubiquinone oxidoreductase,P1
7	j	NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2	polymer	69	7582.6	1	UniProt (Q8LDK3) In PDB	Arabidopsis thaliana (thale cress)
8	k	NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3-A	polymer	72	8064.3	1	UniProt (O64725) Pfam (PF08122) In PDB	Arabidopsis thaliana (thale cress)
9	l	NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial	polymer	125	13225.2	1	UniProt (Q9FGK0) In PDB	Arabidopsis thaliana (thale cress)
10	m	AT2G31490 protein	polymer	71	8305.5	1	UniProt (Q9SIQ8) In PDB	Arabidopsis thaliana (thale cress)	At2g31490,Expressed protein,Neuronal acetylcholine receptor subunit alpha-5,Uncharacterized protein At2g31490,B15 -- 1 beta subcomplex subunit 4
11	n	NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9	polymer	117	13638.3	1	UniProt (Q945M1) In PDB	Arabidopsis thaliana (thale cress)	B22 subunit of eukaryotic mitochondrial complex I,Complex I-B22,CI-B22,NADH-ubiquinone oxidoreductase B22 subunit
12	o	NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7	polymer	103	11757.8	1	UniProt (Q9SKC9) Pfam (PF05676) In PDB	Arabidopsis thaliana (thale cress)
13	p	NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10-B	polymer	106	12462.3	1	UniProt (Q94C12) Pfam (PF10249) In PDB	Arabidopsis thaliana (thale cress)
14	L, M, l	1,2-DIACYL-GLYCEROL-3-SN-PHOSPHATE	non-polymer		705.0	3	Chemie (3PH)
15	L	(1S)-2-{[{[(2R)-2,3-DIHYDROXYPROPYL]OXY}(HYDROXY)PHOSPHORYL]OXY}-1-[(PALMITOYLOXY)METHYL]ETHYL STEARATE	non-polymer		751.0	1	Chemie (PGT)
16	L	PHOSPHATIDYLETHANOLAMINE	non-polymer		734.0	1	Chemie (PTY)
17	L	(7S)-4-HYDROXY-N,N,N-TRIMETHYL-9-OXO-7-[(PALMITOYLOXY)METHYL]-3,5,8-TRIOXA-4-PHOSPHAHEXACOSAN-1-AMINIUM 4-OXIDE	non-polymer		763.1	1	Chemie (PC7)
18	T	S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate	non-polymer		540.7	1	Chemie (8Q1)
19		water	water		18.0	617	Chemie (HOH)

Sequence modifications

L: 1 - 669 (UniProt: P29388)

PDB	External Database	Details
Phe 91	Ser 91	variant
Phe 288	Ser 288	variant
Leu 537	Pro 537	variant

M: 1 - 495 (UniProt: P93313)

PDB	External Database	Details
Phe 146	Pro 146	variant
Leu 326	Pro 326	variant
Phe 383	Ser 383	variant

Contents of the asymmetric unit

Polymers	Number of chains	13
	Total formula weight	253598.3
Non-Polymers*	Number of molecules	7
	Total formula weight	4903.8
All*	Total formula weight	258502.1

*Water molecules are not included.