8AM6
Cyclohexanone dehydrogenase (CDH) from Alicycliphilus denitrificans K601 complexed with dehydrogenated substrate cyclohex-2-en-1-one - inactive mutant (Y195F)
This is a non-PDB format compatible entry.
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | AAA, BaB | Fumarate reductase/succinate dehydrogenase flavoprotein domain protein | polymer | 598 | 64553.6 | 2 | UniProt (F4G7N3) Pfam (PF00890) In PDB | Alicycliphilus denitrificans K601 | |
2 | AAA, BaB | FLAVIN-ADENINE DINUCLEOTIDE | non-polymer | 785.5 | 2 | Chemie (FAD) | |||
3 | BaB, AAA | cyclohex-2-en-1-one | non-polymer | 96.1 | 8 | Chemie (A2Q) | |||
4 | BaB, AAA | GLYCEROL | non-polymer | 92.1 | 27 | Chemie (GOL) | |||
5 | BaB, AAA | SULFATE ION | non-polymer | 96.1 | 9 | Chemie (SO4) | |||
6 | water | water | 18.0 | 1292 | Chemie (HOH) |
Sequence modifications
AAA, BaB: 21 - 598 (UniProt: F4G7N3)
PDB | External Database | Details |
---|---|---|
Met 1 | - | initiating methionine |
Gly 2 | - | expression tag |
Ser 3 | - | expression tag |
Ser 4 | - | expression tag |
His 5 | - | expression tag |
His 6 | - | expression tag |
His 7 | - | expression tag |
His 8 | - | expression tag |
His 9 | - | expression tag |
His 10 | - | expression tag |
Ser 11 | - | expression tag |
Ser 12 | - | expression tag |
Gly 13 | - | expression tag |
Leu 14 | - | expression tag |
Val 15 | - | expression tag |
Pro 16 | - | expression tag |
Arg 17 | - | expression tag |
Gly 18 | - | expression tag |
Ser 19 | - | expression tag |
His 20 | - | expression tag |
Phe 195 | Tyr 176 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 2 |
Total formula weight | 129107.2 | |
Non-Polymers* | Number of molecules | 46 |
Total formula weight | 5691.2 | |
All* | Total formula weight | 134798.4 |