7Z7S

Complex I from E. coli, LMNG-purified, under Turnover at pH 6, Closed state

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	F	NADH-quinone oxidoreductase subunit F	polymer	445	49352.3	1	UniProt (A0A037YNA7) Pfam (PF01512) Pfam (PF10589) UniProt (by SIFTS) (P31979) In PDB	Escherichia coli str. K-12 substr. MC4100
2	E	NADH dehydrogenase I subunit E	polymer	166	18614.0	1	UniProt (A0A829CRH1) Pfam (PF01257) UniProt (by SIFTS) (P0AFD1) In PDB	Escherichia coli str. K-12 substr. MC4100	NADH-quinone oxidoreductase subunit E,NDH-1 subunit E
3	G	NADH-quinone oxidoreductase	polymer	908	100419.2	1	UniProt (A0A037YPU0) Pfam (PF04879) Pfam (PF00384) Pfam (PF01568) Pfam (PF13510) Pfam (PF10588) UniProt (by SIFTS) (P33602) In PDB	Escherichia coli str. K-12 substr. MC4100
4	C	NADH-quinone oxidoreductase subunit C/D	polymer	596	68321.9	1	UniProt (A0A024L1E0) Pfam (PF00329) Pfam (PF00346) UniProt (by SIFTS) (P33599) In PDB	Escherichia coli str. K-12 substr. MC4100	NADH dehydrogenase I subunit C/D,NDH-1 subunit C/D
5	B	NADH-quinone oxidoreductase subunit B	polymer	220	25081.8	1	UniProt (V0ZV11) Pfam (PF01058) UniProt (by SIFTS) (P0AFC7) In PDB	Escherichia coli str. K-12 substr. MC4100	NADH dehydrogenase I subunit B,NDH-1 subunit B
6	I	NADH-quinone oxidoreductase subunit I	polymer	180	20562.8	1	UniProt (A0A1X3LLK1) Pfam (PF00037) UniProt (by SIFTS) (P0AFD6) In PDB	Escherichia coli str. K-12 substr. MC4100
7	H	NADH-quinone oxidoreductase subunit H	polymer	325	36240.9	1	UniProt (E6BKM7) Pfam (PF00146) UniProt (by SIFTS) (P0AFD4) In PDB	Escherichia coli str. K-12 substr. MC4100	NADH dehydrogenase I subunit H,NDH-1 subunit H
8	A	NADH-quinone oxidoreductase subunit A	polymer	147	16474.3	1	UniProt (A0A862ZI24) Pfam (PF00507) UniProt (by SIFTS) (P0AFC3) In PDB	Escherichia coli str. K-12 substr. MC4100	NADH dehydrogenase I subunit A,NDH-1 subunit A,NUO1
9	L	NADH dehydrogenase subunit L	polymer	613	66483.6	1	UniProt (A0A024L0E1) Pfam (PF00662) Pfam (PF00361) UniProt (by SIFTS) (P33607) In PDB	Escherichia coli str. K-12 substr. MC4100	NADH-quinone oxidoreductase subunit L,NADH:ubiquinone oxidoreductase subunit L,NADH:ubiquinone oxidoreductase,membrane subunit L
10	M	NADH dehydrogenase I subunit M	polymer	509	56560.1	1	UniProt (C3T2G7) Pfam (PF00361) UniProt (by SIFTS) (P0AFE8) In PDB	Escherichia coli str. K-12 substr. MC4100	NADH-quinone oxidoreductase subunit M,NDH-1 subunit M
11	N	NADH-quinone oxidoreductase subunit N	polymer	485	52072.7	1	UniProt (E2QPD7) Pfam (PF00361) UniProt (by SIFTS) (P0AFF0) In PDB	Escherichia coli str. K-12 substr. MC4100	NADH dehydrogenase I subunit N,NDH-1 subunit N
12	K	NADH-quinone oxidoreductase subunit K	polymer	100	10853.0	1	UniProt (A0A7U9LRT0) Pfam (PF00420) UniProt (by SIFTS) (P0AFE4) In PDB	Escherichia coli str. K-12 substr. MC4100	NADH dehydrogenase I subunit K,NDH-1 subunit K
13	J	NADH-quinone oxidoreductase subunit J	polymer	184	19889.6	1	UniProt (A0A0H3MIP2) Pfam (PF00499) UniProt (by SIFTS) (P0AFE0) In PDB	Escherichia coli str. K-12 substr. MC4100
14	I, F, G, B	IRON/SULFUR CLUSTER	non-polymer		351.6	7	Chemie (SF4)
15	F	FLAVIN MONONUCLEOTIDE	non-polymer		456.3	1	Chemie (FMN)
16	F	1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE	non-polymer		665.4	1	Chemie (NAI)
17	E, G	FE2/S2 (INORGANIC) CLUSTER	non-polymer		175.8	2	Chemie (FES)
18	G	CALCIUM ION	non-polymer		40.1	1	Chemie (CA)
19	I, H, L, M, N...	1,2-Distearoyl-sn-glycerophosphoethanolamine	non-polymer		748.1	13	Chemie (3PE)
20	C, B	2-decyl-5,6-dimethoxy-3-methylcyclohexa-2,5-diene-1,4-dione	non-polymer		322.4	2	Chemie (DCQ)
21	H, N, J	EICOSANE	non-polymer		282.5	4	Chemie (LFA)
22		water	water		18.0	1184	Chemie (HOH)

Contents of the asymmetric unit

Polymers	Number of chains	13
	Total formula weight	540926.2
Non-Polymers*	Number of molecules	31
	Total formula weight	15474.9
All*	Total formula weight	556401.1

*Water molecules are not included.