7Z7P

Structure of the fluorescent protein NeonCyan0.95 at pH 5.6

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D, E...	NeonCyan0.95	polymer	236	26610.7	8	UniProt (B1PNC0) Pfam (PF01353) UniProt (by SIFTS) (A0A1S4NYF2) In PDB	Branchiostoma lanceolatum (amphioxus)	Modified green fluorescent protein blFP-Y3
2	A, B, C, D, E...	SULFATE ION	non-polymer		96.1	8	Chemie (SO4)
3		water	water		18.0	496	Chemie (HOH)

Sequence modifications

A, B, C, D, E, F, G, H: 12 - 229 (UniProt: B1PNC0)

PDB	External Database	Details
Met 1	-	initiating methionine
Val 2	-	expression tag
Ser 3	-	expression tag
Lys 4	-	expression tag
Gly 5	-	expression tag
Glu 6	-	expression tag
Glu 7	-	expression tag
Asp 8	-	expression tag
Asn 9	-	expression tag
Met 10	-	expression tag
Ala 11	-	expression tag
Ile 25	Phe 15	engineered mutation
Gln 35	Arg 25	engineered mutation
Glu 42	Asp 32	engineered mutation
Asp 55	Ala 45	engineered mutation
His 66	Gln 56	engineered mutation
Val 67	Ile 57	engineered mutation
Io8 69	Gly 58	chromophore
-	Tyr 59	chromophore
-	Gly 60	chromophore
Tyr 77	Phe 67	engineered mutation
Val 89	Lys 79	engineered mutation
Val 110	Ser 100	engineered mutation
Ala 125	Phe 115	engineered mutation
Lys 128	Ile 118	engineered mutation
Val 146	Ala 136	engineered mutation
Ser 148	Trp 138	engineered mutation
Trp 150	Val 140	engineered mutation
Ser 151	Thr 141	engineered mutation
Lys 153	Met 143	engineered mutation
Thr 154	Leu 144	engineered mutation
Lys 166	Asp 156	engineered mutation
Ser 168	Thr 158	engineered mutation
Asn 173	Ser 163	engineered mutation
Glu 175	Lys 165	engineered mutation
Arg 178	Gln 168	engineered mutation
Ala 181	Val 171	engineered mutation
Thr 184	Asn 174	engineered mutation
Tyr 195	Ile 185	engineered mutation
Val 201	Met 191	engineered mutation
Tyr 202	Phe 192	engineered mutation
Glu 213	Lys 203	engineered mutation
Gly 230	-	expression tag
Met 231	-	expression tag
Asp 232	-	expression tag
Glu 233	-	expression tag
Leu 234	-	expression tag
Tyr 235	-	expression tag
Lys 236	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	212885.5
Non-Polymers*	Number of molecules	8
	Total formula weight	768.5
All*	Total formula weight	213654.0

*Water molecules are not included.