7Z03

Endonuclease state of the E. coli Mre11-Rad50 (SbcCD) head complex bound to ADP and extended dsDNA

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	C, D	Nuclease SbcCD subunit C	polymer	1048	118851.5	2	UniProt (P13458) Pfam (PF13476) Pfam (PF13558) In PDB	Escherichia coli
2	A, B	Nuclease SbcCD subunit D	polymer	407	45640.3	2	UniProt (P0AG76) Pfam (PF00149) Pfam (PF12320) In PDB	Escherichia coli
3	E	DNA (39-MER)	polymer	120	37099.6	1	GenBank (1825215262)	synthetic construct
4	F	DNA (39-MER)	polymer	120	36970.5	1	GenBank (1825215262)	synthetic construct
5	C, D	ADENOSINE-5'-DIPHOSPHATE	non-polymer		427.2	2	Chemie (ADP)
6	C, D	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
7	A, B	MANGANESE (II) ION	non-polymer		54.9	4	Chemie (MN)

Sequence modifications

A, B: 1 - 400 (UniProt: P0AG76)

PDB	External Database	Details
Gln 84	His 84	engineered mutation
Gly 401	-	expression tag
His 402	-	expression tag
His 403	-	expression tag
His 404	-	expression tag
His 405	-	expression tag
His 406	-	expression tag
His 407	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	403053.7
Non-Polymers*	Number of molecules	8
	Total formula weight	1122.8
All*	Total formula weight	404176.5

*Water molecules are not included.