7YMZ

Cryo-EM structure of MERS-CoV spike protein, intermediate conformation

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C	Spike glycoprotein	polymer	1369	150654.4	3	UniProt (K0BRG7) Pfam (PF16451) Pfam (PF09408) Pfam (PF19209) Pfam (PF01601) In PDB	Human betacoronavirus 2c EMC/2012	S glycoprotein,E2,Peplomer protein
2	D, F, I, J, L...	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	12	In PDB GlyTouCan (G42666HT)
3	E, M, R	alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		910.8	3	In PDB GlyTouCan (G22768VO)
4	G, H, K, O, S...	alpha-L-fucopyranose-(1-6)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		367.3	6	In PDB GlyTouCan (G86851RC)
5	A, B, C	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	12	Chemie (NAG)

Sequence modifications

A, B, C: 17 - 1291 (UniProt: K0BRG7)

PDB	External Database	Details
Met -2	-	initiating methionine
Asp -1	-	expression tag
Ser 0	-	expression tag
Trp 1	-	expression tag
Phe 2	-	expression tag
Ile 3	-	expression tag
Leu 4	-	expression tag
Val 5	-	expression tag
Leu 6	-	expression tag
Leu 7	-	expression tag
Gly 8	-	expression tag
Ser 9	-	expression tag
Gly 10	-	expression tag
Leu 11	-	expression tag
Ile 12	-	expression tag
Cys 13	-	expression tag
Val 14	-	expression tag
Ser 15	-	expression tag
Ala 16	-	expression tag
Ala 748	Arg 748	engineered mutation
Gly 751	Arg 751	engineered mutation
Pro 1060	Val 1060	engineered mutation
Pro 1061	Leu 1061	engineered mutation
Glu 1292	-	expression tag
Phe 1293	-	expression tag
Gly 1294	-	expression tag
Ser 1295	-	expression tag
Gly 1296	-	expression tag
Gly 1297	-	expression tag
Tyr 1298	-	expression tag
Ile 1299	-	expression tag
Pro 1300	-	expression tag
Glu 1301	-	expression tag
Ala 1302	-	expression tag
Pro 1303	-	expression tag
Arg 1304	-	expression tag
Asp 1305	-	expression tag
Gly 1306	-	expression tag
Gln 1307	-	expression tag
Ala 1308	-	expression tag
Tyr 1309	-	expression tag
Val 1310	-	expression tag
Arg 1311	-	expression tag
Lys 1312	-	expression tag
Asp 1313	-	expression tag
Gly 1314	-	expression tag
Glu 1315	-	expression tag
Trp 1316	-	expression tag
Val 1317	-	expression tag
Leu 1318	-	expression tag
Leu 1319	-	expression tag
Ser 1320	-	expression tag
Thr 1321	-	expression tag
Phe 1322	-	expression tag
Leu 1323	-	expression tag
Lys 1324	-	expression tag
Gly 1325	-	expression tag
Gln 1326	-	expression tag
Asp 1327	-	expression tag
Asn 1328	-	expression tag
Ser 1329	-	expression tag
Ala 1330	-	expression tag
Asp 1331	-	expression tag
Ile 1332	-	expression tag
Gln 1333	-	expression tag
His 1334	-	expression tag
Ser 1335	-	expression tag
Gly 1336	-	expression tag
Arg 1337	-	expression tag
Pro 1338	-	expression tag
Leu 1339	-	expression tag
Glu 1340	-	expression tag
Ser 1341	-	expression tag
Arg 1342	-	expression tag
Gly 1343	-	expression tag
Pro 1344	-	expression tag
Phe 1345	-	expression tag
Glu 1346	-	expression tag
Gln 1347	-	expression tag
Lys 1348	-	expression tag
Leu 1349	-	expression tag
Ile 1350	-	expression tag
Ser 1351	-	expression tag
Glu 1352	-	expression tag
Glu 1353	-	expression tag
Asp 1354	-	expression tag
Leu 1355	-	expression tag
Asn 1356	-	expression tag
Met 1357	-	expression tag
His 1358	-	expression tag
Thr 1359	-	expression tag
Gly 1360	-	expression tag
His 1361	-	expression tag
His 1362	-	expression tag
His 1363	-	expression tag
His 1364	-	expression tag
His 1365	-	expression tag
His 1366	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	451963.3
Branched	Number of molecules	21
	Total formula weight	10029.4
Non-Polymers*	Number of molecules	12
	Total formula weight	2654.5
All*	Total formula weight	464647.2

*Water molecules are not included.