7X8R
Cryo-EM structure of the Boc5-bound hGLP-1R-Gs complex
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A | Guanine nucleotide-binding protein G(s) subunit alpha isoforms short | polymer | 394 | 45683.4 | 1 | UniProt (P63092) Pfam (PF00503) UniProt (by SIFTS) (P04896) In PDB | Bos taurus (cattle) | Adenylate cyclase-stimulating G alpha protein |
2 | B | Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 | polymer | 345 | 37915.5 | 1 | UniProt (P54311) Pfam (PF00400) In PDB | Rattus norvegicus (Norway rat) | Transducin beta chain 1 |
3 | G | Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 | polymer | 70 | 7729.9 | 1 | UniProt (P59768) Pfam (PF00631) In PDB | Homo sapiens (human) | G gamma-I |
4 | N | Nanobody 35 | polymer | 140 | 15343.0 | 1 | synthetic construct | ||
5 | R | Glucagon-like peptide 1 receptor | polymer | 440 | 50894.8 | 1 | UniProt (P43220) Pfam (PF02793) Pfam (PF00002) In PDB | Homo sapiens (human) | GLP-1 receptor,GLP-1-R,GLP-1R |
6 | R | 2,4-bis(3-methoxy-4-thiophen-2-ylcarbonyloxy-phenyl)-1,3-bis[[4-[(2-methylpropan-2-yl)oxycarbonylamino]phenyl]carbonylamino]cyclobutane-1,3-dicarboxylic acid | non-polymer | 1077.1 | 1 | Chemie (BYI) |
Sequence modifications
A: 1 - 394 (UniProt: P63092)
B: 2 - 340 (UniProt: P54311)
R: 24 - 463 (UniProt: P43220)
PDB | External Database | Details |
---|---|---|
Asn 54 | Ser 54 | conflict |
Ala 226 | Gly 226 | conflict |
Ala 268 | Glu 268 | conflict |
Lys 271 | Asn 271 | conflict |
Asp 274 | Lys 274 | conflict |
Lys 280 | Arg 280 | variant |
Asp 284 | Thr 284 | conflict |
Thr 285 | Ile 285 | conflict |
PDB | External Database | Details |
---|---|---|
Met -4 | - | initiating methionine |
Gly -3 | - | expression tag |
Ser -2 | - | expression tag |
Leu -1 | - | expression tag |
Leu 0 | - | expression tag |
Gln 1 | - | expression tag |
PDB | External Database | Details |
---|---|---|
Phe 260 | Leu 260 | variant |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 5 |
Total formula weight | 157566.7 | |
Non-Polymers* | Number of molecules | 1 |
Total formula weight | 1077.1 | |
All* | Total formula weight | 158643.9 |