7WZ4

Structure of an orphan GPCR-G protein signaling complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	R	Probable G-protein coupled receptor 88	polymer	520	55697.9	1	UniProt (Q9GZN0) Pfam (PF00001) In PDB	Homo sapiens (human)	Striatum-specific G-protein coupled receptor
2	A	Guanine nucleotide-binding protein G(i) subunit alpha-1	polymer	354	40415.0	1	UniProt (P63096) Pfam (PF00503) In PDB	Homo sapiens (human)	Adenylate cyclase-inhibiting G alpha protein
3	B	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	357	39286.9	1	UniProt (P62873) Pfam (PF00400) In PDB	Homo sapiens (human)	Transducin beta chain 1
4	G	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7861.1	1	UniProt (P59768) Pfam (PF00631) In PDB	Homo sapiens (human)	G gamma-I
5	S	scFv16	polymer	259	27784.9	1		Mus musculus

Sequence modifications

R: 1 - 384 (UniProt: Q9GZN0)

PDB	External Database	Details
Asp -123	-	expression tag
Tyr -122	-	expression tag
Lys -121	-	expression tag
Asp -120	-	expression tag
Asp -119	-	expression tag
Asp -118	-	expression tag
Asp -117	-	expression tag
Lys -116	-	expression tag
Leu -115	-	expression tag
Glu -114	-	expression tag
Val -113	-	expression tag
Leu -112	-	expression tag
Phe -111	-	expression tag
Gln -110	-	expression tag
Gly -109	-	expression tag
Pro -108	-	expression tag
Gly -107	-	expression tag
Ser -106	-	expression tag
Ala -105	-	expression tag
Asp -104	-	expression tag
Leu -103	-	expression tag
Glu -102	-	expression tag
Asp -101	-	expression tag
Asn -100	-	expression tag
Trp -99	-	expression tag
Glu -98	-	expression tag
Thr -97	-	expression tag
Leu -96	-	expression tag
Asn -95	-	expression tag
Asp -94	-	expression tag
Asn -93	-	expression tag
Leu -92	-	expression tag
Lys -91	-	expression tag
Val -90	-	expression tag
Ile -89	-	expression tag
Glu -88	-	expression tag
Lys -87	-	expression tag
Ala -86	-	expression tag
Asp -85	-	expression tag
Asn -84	-	expression tag
Ala -83	-	expression tag
Ala -82	-	expression tag
Gln -81	-	expression tag
Val -80	-	expression tag
Lys -79	-	expression tag
Asp -78	-	expression tag
Ala -77	-	expression tag
Leu -76	-	expression tag
Thr -75	-	expression tag
Lys -74	-	expression tag
Met -73	-	expression tag
Arg -72	-	expression tag
Ala -71	-	expression tag
Ala -70	-	expression tag
Ala -69	-	expression tag
Leu -68	-	expression tag
Asp -67	-	expression tag
Ala -66	-	expression tag
Gln -65	-	expression tag
Lys -64	-	expression tag
Ala -63	-	expression tag
Thr -62	-	expression tag
Pro -61	-	expression tag
Pro -60	-	expression tag
Lys -59	-	expression tag
Leu -58	-	expression tag
Glu -57	-	expression tag
Asp -56	-	expression tag
Lys -55	-	expression tag
Ser -54	-	expression tag
Pro -53	-	expression tag
Asp -52	-	expression tag
Ser -51	-	expression tag
Pro -50	-	expression tag
Glu -49	-	expression tag
Met -48	-	expression tag
Lys -47	-	expression tag
Asp -46	-	expression tag
Phe -45	-	expression tag
Arg -44	-	expression tag
His -43	-	expression tag
Gly -42	-	expression tag
Phe -41	-	expression tag
Asp -40	-	expression tag
Ile -39	-	expression tag
Leu -38	-	expression tag
Val -37	-	expression tag
Gly -36	-	expression tag
Gln -35	-	expression tag
Ile -34	-	expression tag
Asp -33	-	expression tag
Asp -32	-	expression tag
Ala -31	-	expression tag
Leu -30	-	expression tag
Lys -29	-	expression tag
Leu -28	-	expression tag
Ala -27	-	expression tag
Asn -26	-	expression tag
Glu -25	-	expression tag
Gly -24	-	expression tag
Lys -23	-	expression tag
Val -22	-	expression tag
Lys -21	-	expression tag
Glu -20	-	expression tag
Ala -19	-	expression tag
Gln -18	-	expression tag
Ala -17	-	expression tag
Ala -16	-	expression tag
Ala -15	-	expression tag
Glu -14	-	expression tag
Gln -13	-	expression tag
Leu -12	-	expression tag
Lys -11	-	expression tag
Thr -10	-	expression tag
Thr -9	-	expression tag
Arg -8	-	expression tag
Asn -7	-	expression tag
Ala -6	-	expression tag
Tyr -5	-	expression tag
Ile -4	-	expression tag
Gln -3	-	expression tag
Lys -2	-	expression tag
Tyr -1	-	expression tag
Leu 0	-	expression tag
Leu 385	-	expression tag
Glu 386	-	expression tag
His 387	-	expression tag
His 388	-	expression tag
His 389	-	expression tag
His 390	-	expression tag
His 391	-	expression tag
His 392	-	expression tag
His 393	-	expression tag
His 394	-	expression tag
His 395	-	expression tag
His 396	-	expression tag

B: 2 - 340 (UniProt: P62873)

PDB	External Database	Details
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
Leu -10	-	expression tag
Glu -9	-	expression tag
Val -8	-	expression tag
Leu -7	-	expression tag
Phe -6	-	expression tag
Gln -5	-	expression tag
Gly -4	-	expression tag
Pro -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
Ser 0	-	expression tag
Gly 1	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	171045.9
All*	Total formula weight	171045.9

*Water molecules are not included.