7W6P

Cryo-EM structure of the alpha2A adrenergic receptor GoA signaling complex bound to a G protein biased agonist

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Guanine nucleotide-binding protein G(o) subunit alpha	polymer	354	40100.5	1	UniProt (P09471) Pfam (PF00503) In PDB	Homo sapiens (human)
2	B	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	349	38402.9	1	UniProt (P62873) Pfam (PF00400) In PDB	Homo sapiens (human)	Transducin beta chain 1
3	G	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7861.1	1	UniProt (P59768) Pfam (PF00631) In PDB	Homo sapiens (human)	G gamma-I
4	H	scFv	polymer	307	32898.8	1		Mus musculus (mouse)
5	R	Alpha-2A adrenergic receptor	polymer	465	50704.6	1	UniProt (P08913) Pfam (PF00001) In PDB	Homo sapiens (human)	Alpha-2 adrenergic receptor subtype C10,Alpha-2A adrenoreceptor,Alpha-2A adrenoceptor,Alpha-2AAR
6	R	N-pyridin-4-ylisoquinolin-4-amine	non-polymer		221.3	1	Chemie (W96)

Sequence modifications

B: 2 - 340 (UniProt: P62873)

PDB	External Database	Details
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
Ser 0	-	expression tag
Gly 1	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	169967.9
Non-Polymers*	Number of molecules	1
	Total formula weight	221.3
All*	Total formula weight	170189.1

*Water molecules are not included.