7UXC

cryo-EM structure of the mTORC1-TFEB-Rag-Ragulator complex with symmetry expansion

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Serine/threonine-protein kinase mTOR	polymer	2549	289258.0	1	UniProt (P42345) Pfam (PF11865) Pfam (PF02259) Pfam (PF08771) Pfam (PF00454) Pfam (PF02260) In PDB	Homo sapiens (human)	FK506-binding protein 12-rapamycin complex-associated protein 1,FKBP12-rapamycin complex-associated protein,Mammalian target of rapamycin,mTOR,Mechanistic target of rapamycin,Rapamycin and FKBP12 target 1,Rapamycin target protein 1
2	B	Target of rapamycin complex subunit LST8	polymer	326	35910.1	1	UniProt (Q9BVC4) Pfam (PF00400) In PDB	Homo sapiens (human)	TORC subunit LST8,G protein beta subunit-like,Protein GbetaL,Mammalian lethal with SEC13 protein 8,mLST8
3	C	Regulatory-associated protein of mTOR	polymer	1335	149200.0	1	UniProt (Q8N122) Pfam (PF14538) Pfam (PF02985) Pfam (PF00400) In PDB	Homo sapiens (human)	Raptor,p150 target of rapamycin (TOR)-scaffold protein
4	D, K	Ras-related GTP-binding protein A	polymer	313	36600.2	2	UniProt (Q7L523) Pfam (PF04670) In PDB	Homo sapiens (human)	Rag A,RagA,Adenovirus E3 14.7 kDa-interacting protein 1,FIP-1
5	E, L	Ras-related GTP-binding protein C	polymer	399	44298.9	2	UniProt (Q9HB90) Pfam (PF04670) In PDB	Homo sapiens (human)	RagC,GTPase-interacting protein 2,TIB929
6	F, M	Ragulator complex protein LAMTOR1	polymer	161	17762.8	2	UniProt (Q6IAA8) Pfam (PF15454) In PDB	Homo sapiens (human)	Late endosomal/lysosomal adaptor and MAPK and MTOR activator 1,Lipid raft adaptor protein p18,Protein associated with DRMs and endosomes,p27Kip1-releasing factor from RhoA,p27RF-Rho
7	G, N	Ragulator complex protein LAMTOR2	polymer	125	13517.5	2	UniProt (Q9Y2Q5) Pfam (PF03259) In PDB	Homo sapiens (human)	Endosomal adaptor protein p14,Late endosomal/lysosomal Mp1-interacting protein,Late endosomal/lysosomal adaptor and MAPK and MTOR activator 2,Mitogen-activated protein-binding protein-interacting protein,MAPBP-interacting protein,Roadblock domain-containing protein 3
8	H, O	Ragulator complex protein LAMTOR3	polymer	124	13637.7	2	UniProt (Q9UHA4) Pfam (PF08923) In PDB	Homo sapiens (human)	Late endosomal/lysosomal adaptor and MAPK and MTOR activator 3,MEK-binding partner 1,Mp1,Mitogen-activated protein kinase kinase 1-interacting protein 1,Mitogen-activated protein kinase scaffold protein 1
9	I, P	Ragulator complex protein LAMTOR4	polymer	99	10753.2	2	UniProt (Q0VGL1) In PDB	Homo sapiens (human)	Late endosomal/lysosomal adaptor and MAPK and MTOR activator 4
10	J, Q	Ragulator complex protein LAMTOR5	polymer	91	9622.9	2	UniProt (O43504) Pfam (PF16672) In PDB	Homo sapiens (human)	Hepatitis B virus X-interacting protein,HBX-interacting protein,Late endosomal/lysosomal adaptor and MAPK and MTOR activator 5
11	R	Transcription factor EB	polymer	476	52926.6	1	UniProt (P19484) Pfam (PF15951) Pfam (PF00010) Pfam (PF11851) In PDB	Homo sapiens (human)	Class E basic helix-loop-helix protein 35,bHLHe35
12	A	INOSITOL HEXAKISPHOSPHATE	non-polymer		660.0	1	Chemie (IHP)
13	D, K	GUANOSINE-5'-TRIPHOSPHATE	non-polymer		523.2	2	Chemie (GTP)
14	D, K	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
15	E, L	GUANOSINE-5'-DIPHOSPHATE	non-polymer		443.2	2	Chemie (GDP)

Sequence modifications

D, K: 1 - 313 (UniProt: Q7L523)

PDB	External Database	Details
Leu 66	Gln 66	conflict

E, L: 1 - 399 (UniProt: Q9HB90)

PDB	External Database	Details
Asn 75	Ser 75	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	18
	Total formula weight	819680.9
Non-Polymers*	Number of molecules	7
	Total formula weight	2641.4
All*	Total formula weight	822322.3

*Water molecules are not included.