7UNF

CryoEM structure of a mEAK7 bound human V-ATPase complex

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	a	V-type proton ATPase 116 kDa subunit a 4	polymer	856	98530.5	1	UniProt (Q9HBG4) Pfam (PF01496) In PDB	Homo sapiens (human)	V-ATPase 116 kDa isoform a 4,Vacuolar proton translocating ATPase 116 kDa subunit a isoform 4,Vacuolar proton translocating ATPase 116 kDa subunit a kidney isoform
2	U	KIAA1609 protein, isoform CRA_a	polymer	456	51096.5	1	UniProt (D3DUL8) In PDB	Homo sapiens (human)
3	L, M, N	V-type proton ATPase catalytic subunit A	polymer	617	68379.9	3	UniProt (P38606) Pfam (PF02874) Pfam (PF16886) Pfam (PF00006) In PDB	Homo sapiens (human)	V-ATPase subunit A,V-ATPase 69 kDa subunit,Vacuolar ATPase isoform VA68,Vacuolar proton pump subunit alpha
4	O, P, Q	V-type proton ATPase subunit B, brain isoform	polymer	511	56561.5	3	UniProt (P21281) Pfam (PF02874) Pfam (PF00006) In PDB	Homo sapiens (human)	V-ATPase subunit B 2,Endomembrane proton pump 58 kDa subunit,HO57,Vacuolar proton pump subunit B 2
5	D	V-type proton ATPase subunit D	polymer	247	28311.9	1	UniProt (Q9Y5K8) Pfam (PF01813) In PDB	Homo sapiens (human)	V-ATPase subunit D,V-ATPase 28 kDa accessory protein,Vacuolar proton pump subunit D
6	b, c, d	V-type proton ATPase subunit E 1	polymer	226	26183.3	3	UniProt (P36543) Pfam (PF01991) In PDB	Homo sapiens (human)	V-ATPase subunit E 1,V-ATPase 31 kDa subunit,p31,Vacuolar proton pump subunit E 1
7	e, f, g	V-type proton ATPase subunit G 1	polymer	118	13781.5	3	UniProt (O75348) Pfam (PF03179) In PDB	Homo sapiens (human)	V-ATPase subunit G 1,V-ATPase 13 kDa subunit 1,Vacuolar proton pump subunit G 1,Vacuolar proton pump subunit M16
8	F	V-type proton ATPase subunit F	polymer	119	13388.2	1	UniProt (Q16864) Pfam (PF01990) In PDB	Homo sapiens (human)	V-ATPase subunit F,V-ATPase 14 kDa subunit,Vacuolar proton pump subunit F
9	C	V-type proton ATPase subunit C 1	polymer	382	43999.5	1	UniProt (P21283) Pfam (PF03223) In PDB	Homo sapiens (human)	V-ATPase subunit C 1,Vacuolar proton pump subunit C 1
10	H	V-type proton ATPase subunit H	polymer	483	55949.9	1	UniProt (Q9UI12) Pfam (PF03224) Pfam (PF11698) In PDB	Homo sapiens (human)	V-ATPase subunit H,Nef-binding protein 1,NBP1,Protein VMA13 homolog,V-ATPase 50/57 kDa subunits,Vacuolar proton pump subunit H,Vacuolar proton pump subunit SFD
11	k	V-type proton ATPase subunit d 1	polymer	351	40369.9	1	UniProt (P61421) Pfam (PF01992) In PDB	Homo sapiens (human)	V-ATPase subunit d 1,32 kDa accessory protein,V-ATPase 40 kDa accessory protein,V-ATPase AC39 subunit,p39,Vacuolar proton pump subunit d 1
12	m	V-type proton ATPase subunit e 1	polymer	81	9380.3	1	UniProt (O15342) Pfam (PF05493) In PDB	Homo sapiens (human)	V-ATPase subunit e 1,V-ATPase 9.2 kDa membrane accessory protein,V-ATPase M9.2 subunit,Vacuolar proton pump subunit e 1
13	n	Ribonuclease kappa	polymer	137	15435.2	1	UniProt (Q6P5S7) In PDB	Homo sapiens (human)	RNase kappa
14	s	V-type proton ATPase subunit S1	polymer	470	52067.5	1	UniProt (Q15904) Pfam (PF05827) Pfam (PF20520) In PDB	Homo sapiens (human)	V-ATPase subunit S1,Protein XAP-3,V-ATPase Ac45 subunit,V-ATPase S1 accessory protein,Vacuolar proton pump subunit S1
15	r	ATPase H(+)-transporting lysosomal accessory protein 2	polymer	345	38421.1	1	UniProt (A0A1B0GVW0) Pfam (PF07850) In PDB	Homo sapiens (human)	ATPase H(+)-transporting lysosomal-interacting protein 2,Renin receptor,Renin/prorenin receptor
16	8, 9, 2, 3, 4...	V-type proton ATPase 16 kDa proteolipid subunit	polymer	155	15743.7	9	UniProt (P27449) Pfam (PF00137) In PDB	Homo sapiens (human)	V-ATPase 16 kDa proteolipid subunit,Vacuolar proton pump 16 kDa proteolipid subunit
17	1	V-type proton ATPase 21 kDa proteolipid subunit	polymer	205	21418.2	1	UniProt (Q99437) Pfam (PF00137) In PDB	Homo sapiens (human)	V-ATPase 21 kDa proteolipid subunit,Vacuolar proton pump 21 kDa proteolipid subunit,hATPL
18	t	alpha-D-glucopyranose-(1-3)-alpha-D-glucopyranose-(1-3)-alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		1397.2	1	In PDB
19	w	alpha-D-mannopyranose-(1-6)-alpha-D-mannopyranose	branched		342.3	1	In PDB GlyTouCan (G77384RA)
20	M	ADENOSINE-5'-DIPHOSPHATE	non-polymer		427.2	1	Chemie (ADP)
21	s	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	6	Chemie (NAG)
22	s, r, 8, 1	(2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate	non-polymer		760.1	9	Chemie (POV)

Sequence modifications

a: 1 - 840 (UniProt: Q9HBG4)

PDB	External Database	Details
Arg 252	Pro 252	conflict
Asp 841	-	expression tag
Tyr 842	-	expression tag
Lys 843	-	expression tag
Asp 844	-	expression tag
Asp 845	-	expression tag
Asp 846	-	expression tag
Asp 847	-	expression tag
Lys 848	-	expression tag
Asp 849	-	expression tag
Tyr 850	-	expression tag
Lys 851	-	expression tag
Asp 852	-	expression tag
Asp 853	-	expression tag
Asp 854	-	expression tag
Asp 855	-	expression tag
Lys 856	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	33
	Total formula weight	1104780.6
Branched	Number of molecules	2
	Total formula weight	1739.5
Non-Polymers*	Number of molecules	16
	Total formula weight	8595.1
All*	Total formula weight	1115115.3

*Water molecules are not included.