7UND
Pol II-DSIF-SPT6-PAF1c-TFIIS-nucleosome complex (stalled at +38)
This is a non-PDB format compatible entry.
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A | DNA-directed RNA polymerase subunit | polymer | 1984 | 219049.5 | 1 | UniProt (A0A7M4DUC2) Pfam (PF04997) Pfam (PF05001) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998) Pfam (PF04992) Pfam (PF04990) In PDB | Sus scrofa (pig) | |
2 | B | DNA-directed RNA polymerase subunit beta | polymer | 1251 | 142426.1 | 1 | UniProt (A0A4X1TVZ5) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF04566) Pfam (PF04567) Pfam (PF00562) Pfam (PF04560) In PDB | Sus scrofa (pig) | |
3 | C | DNA-directed RNA polymerase II subunit RPB3 | polymer | 275 | 31439.1 | 1 | UniProt (A0A481DF93) Pfam (PF01193) Pfam (PF01000) In PDB | Sus scrofa (pig) | |
4 | D | RPOL4c domain-containing protein | polymer | 184 | 20962.6 | 1 | UniProt (A0A8D1GCS3) Pfam (PF03874) In PDB | Sus scrofa (pig) | |
5 | E | DNA-directed RNA polymerase II subunit E | polymer | 210 | 24644.3 | 1 | UniProt (A0A4X1VTX4) Pfam (PF03871) Pfam (PF01191) In PDB | Sus scrofa (pig) | RPB5 |
6 | F | DNA-directed RNA polymerases I, II, and III subunit RPABC2 | polymer | 127 | 14477.0 | 1 | UniProt (A0A4X1VEK9) Pfam (PF01192) In PDB | Sus scrofa (pig) | DNA-directed RNA polymerase II subunit F,RPB6 homolog |
7 | G | DNA-directed RNA polymerase II subunit RPB7 | polymer | 172 | 19314.3 | 1 | UniProt (A0A4X1VKG7) Pfam (PF03876) Pfam (PF00575) In PDB | Sus scrofa (pig) | |
8 | H | RPB8 | polymer | 150 | 17162.3 | 1 | Sus scrofa | ||
9 | I | RPB9 | polymer | 125 | 14541.2 | 1 | Sus scrofa | ||
10 | J | RPB10 | polymer | 67 | 7655.1 | 1 | Sus scrofa | ||
11 | K | RPB11 | polymer | 117 | 13310.3 | 1 | Sus scrofa | ||
12 | L | RNA polymerase II subunit K | polymer | 58 | 7018.2 | 1 | UniProt (A0A4X1TRS6) Pfam (PF03604) In PDB | Sus scrofa (pig) | |
13 | M | Transcription elongation factor SPT6 | polymer | 1729 | 199603.0 | 1 | UniProt (Q7KZ85) Pfam (PF14632) Pfam (PF14641) Pfam (PF14639) Pfam (PF14635) Pfam (PF17674) Pfam (PF00575) Pfam (PF14633) In PDB | Homo sapiens (human) | hSPT6,Histone chaperone suppressor of Ty6,Tat-cotransactivator 2 protein,Tat-CT2 protein |
14 | N | Non-template DNA | polymer | 209 | 64763.0 | 1 | synthetic construct | ||
15 | O | Transcription elongation factor A protein 1 | polymer | 304 | 34295.0 | 1 | UniProt (P23193) Pfam (PF08711) Pfam (PF07500) Pfam (PF01096) In PDB | Homo sapiens (human) | TFIIS |
16 | P | RNA | polymer | 18 | 5735.4 | 1 | synthetic construct | ||
17 | Q | RNA polymerase-associated protein CTR9 homolog | polymer | 1179 | 134510.2 | 1 | UniProt (Q6PD62) Pfam (PF13374) Pfam (PF13181) In PDB | Homo sapiens (human) | SH2 domain-binding protein 1 |
18 | R | RNA polymerase-associated protein RTF1 homolog | polymer | 713 | 80733.0 | 1 | UniProt (Q92541) Pfam (PF03126) In PDB | Homo sapiens (human) | |
19 | T | Template DNA | polymer | 215 | 66186.4 | 1 | synthetic construct | ||
20 | U | RNA polymerase-associated protein LEO1 | polymer | 666 | 75514.2 | 1 | UniProt (Q8WVC0) Pfam (PF04004) In PDB | Homo sapiens (human) | Replicative senescence down-regulated leo1-like protein |
21 | V | RNA polymerase II-associated factor 1 homolog | polymer | 531 | 60052.7 | 1 | UniProt (Q8N7H5) Pfam (PF03985) In PDB | Homo sapiens (human) | hPAF1,Pancreatic differentiation protein 2 |
22 | W | WDR61 | polymer | 305 | 33617.5 | 1 | UniProt (Q9GZS3) Pfam (PF00400) In PDB | Homo sapiens (human) | |
23 | X | Parafibromin | polymer | 531 | 60673.5 | 1 | UniProt (Q6P1J9) Pfam (PF16050) Pfam (PF05179) In PDB | Homo sapiens (human) | Cell division cycle protein 73 homolog,Hyperparathyroidism 2 protein |
24 | Y | Transcription elongation factor SPT4 | polymer | 117 | 13210.2 | 1 | UniProt (P63272) Pfam (PF06093) In PDB | Homo sapiens (human) | hSPT4,DRB sensitivity-inducing factor 14 kDa subunit,DSIF p14,DRB sensitivity-inducing factor small subunit,DSIF small subunit |
25 | Z | Transcription elongation factor SPT5 | polymer | 1087 | 121225.5 | 1 | UniProt (O00267) Pfam (PF11942) Pfam (PF03439) Pfam (PF00467) In PDB | Homo sapiens (human) | hSPT5,DRB sensitivity-inducing factor 160 kDa subunit,DSIF p160,DRB sensitivity-inducing factor large subunit,DSIF large subunit,Tat-cotransactivator 1 protein,Tat-CT1 protein |
26 | a, e | Histone H3.2 | polymer | 136 | 15435.1 | 2 | UniProt (P84233) Pfam (PF00125) In PDB | Xenopus laevis (African clawed frog) | Histone H3 |
27 | b, f | Histone H4 | polymer | 103 | 11394.4 | 2 | UniProt (P62799) Pfam (PF15511) In PDB | Xenopus laevis (African clawed frog) | |
28 | c, g | Histone H2A | polymer | 130 | 14109.4 | 2 | UniProt (Q6AZJ8) Pfam (PF00125) Pfam (PF16211) In PDB | Xenopus laevis (African clawed frog) | |
29 | d, h | Histone H2B 1.1 | polymer | 123 | 13655.9 | 2 | UniProt (P02281) Pfam (PF00125) In PDB | Xenopus laevis (African clawed frog) | H2B1.1 |
30 | A, B, C, I, J... | ZINC ION | non-polymer | 65.4 | 9 | Chemie (ZN) | |||
31 | A | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) |
Sequence modifications
M: 1 - 1723 (UniProt: Q7KZ85)
O: 1 - 301 (UniProt: P23193)
Q: 1 - 1173 (UniProt: Q6PD62)
R: 1 - 710 (UniProt: Q92541)
a, e: 0 - 135 (UniProt: P84233)
d, h: 1 - 122 (UniProt: P02281)
PDB | External Database | Details |
---|---|---|
Ser -2 | - | expression tag |
Asn -1 | - | expression tag |
Ala 0 | - | expression tag |
PDB | External Database | Details |
---|---|---|
Ser -2 | - | expression tag |
Asn -1 | - | expression tag |
Ala 0 | - | expression tag |
PDB | External Database | Details |
---|---|---|
Glu 1174 | - | expression tag |
Asn 1175 | - | expression tag |
Leu 1176 | - | expression tag |
Tyr 1177 | - | expression tag |
Phe 1178 | - | expression tag |
Gln 1179 | - | expression tag |
PDB | External Database | Details |
---|---|---|
Ser -2 | - | expression tag |
Asn -1 | - | expression tag |
Ala 0 | - | expression tag |
PDB | External Database | Details |
---|---|---|
Ala 102 | Gly 103 | engineered mutation |
PDB | External Database | Details |
---|---|---|
Met 0 | - | initiating methionine |
Thr 29 | Ser 33 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 33 |
Total formula weight | 1591309.4 | |
Non-Polymers* | Number of molecules | 10 |
Total formula weight | 613.0 | |
All* | Total formula weight | 1591922.4 |