7UFU

Cryo-EM Structure of Bl_Man38A nucleophile mutant in complex with mannose at 2.7 A

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D	Alpha-mannosidase	polymer	1062	117943.1	4	UniProt (Q8G4Q1) Pfam (PF01074) Pfam (PF09261) Pfam (PF07748) Pfam (PF17677) In PDB	Bifidobacterium longum
2	A, B, C, D	ZINC ION	non-polymer		65.4	4	Chemie (ZN)
3	A, B, C, D	alpha-D-mannopyranose	non-polymer		180.2	4	Chemie (MAN)

Sequence modifications

A, B, C, D: 1 - 1039 (UniProt: Q8G4Q1)

PDB	External Database	Details
Met -22	-	initiating methionine
Gly -21	-	expression tag
Ser -20	-	expression tag
Ser -19	-	expression tag
His -18	-	expression tag
His -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
Ser -12	-	expression tag
Ser -11	-	expression tag
Gly -10	-	expression tag
Leu -9	-	expression tag
Val -8	-	expression tag
Pro -7	-	expression tag
Arg -6	-	expression tag
Gly -5	-	expression tag
Ser -4	-	expression tag
His -3	-	expression tag
Met -2	-	expression tag
Ala -1	-	expression tag
Ser 0	-	expression tag
Ala 387	Asp 387	conflict

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	471772.5
Non-Polymers*	Number of molecules	8
	Total formula weight	982.3
All*	Total formula weight	472754.8

*Water molecules are not included.