7TYY

Human Amylin2 Receptor in complex with Gs and salmon calcitonin peptide

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	E	Receptor activity-modifying protein 2	polymer	156	17839.4	1	UniProt (O60895) Pfam (PF04901) In PDB	Homo sapiens (human)	Calcitonin-receptor-like receptor activity-modifying protein 2,CRLR activity-modifying protein 2
2	P	Calcitonin-1	polymer	33	3436.9	1	UniProt (B5XGR7) In PDB	Salmo salar	Calcitonin-1 precursor,calcitonin-1-like isoform X1
3	R	Calcitonin receptor	polymer	501	58469.6	1	UniProt (P30988) Pfam (PF02793) Pfam (PF00002) In PDB	Homo sapiens (human)	CT-R
4	A	Guanine nucleotide-binding protein G(s) subunit alpha isoforms short	polymer	394	45699.4	1	UniProt (P63092) Pfam (PF00503) In PDB	Homo sapiens (human)	Adenylate cyclase-stimulating G alpha protein
5	B	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	350	38534.1	1	UniProt (P62873) Pfam (PF00400) In PDB	Homo sapiens (human)	Transducin beta chain 1
6	G	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7861.1	1	UniProt (P59768) Pfam (PF00631) In PDB	Homo sapiens (human)	G gamma-I
7	N	nanobody 35	polymer	138	15140.7	1		Lama glama (Llama)
8	R	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	1	Chemie (NAG)
9	R	PALMITIC ACID	non-polymer		256.4	5	Chemie (PLM)
10	R	CHOLESTEROL HEMISUCCINATE	non-polymer		486.7	2	Chemie (Y01)
11		water	water		18.0	7	Chemie (HOH)

Sequence modifications

E: 44 - 175 (UniProt: O60895)

PDB	External Database	Details
Met 20	-	expression tag
Lys 21	-	expression tag
Thr 22	-	expression tag
Ile 23	-	expression tag
Ile 24	-	expression tag
Ala 25	-	expression tag
Leu 26	-	expression tag
Ser 27	-	expression tag
Tyr 28	-	expression tag
Ile 29	-	expression tag
Phe 30	-	expression tag
Cys 31	-	expression tag
Leu 32	-	expression tag
Val 33	-	expression tag
Phe 34	-	expression tag
Ala 35	-	expression tag
Asp 36	-	expression tag
Tyr 37	-	expression tag
Lys 38	-	expression tag
Asp 39	-	expression tag
Asp 40	-	expression tag
Asp 41	-	expression tag
Asp 42	-	expression tag
Lys 43	-	expression tag

P: 1 - 32 (UniProt: B5XGR7)

PDB	External Database	Details
Nh2 33	-	amidation

R: 25 - 474 (UniProt: P30988)

PDB	External Database	Details
Met -7	-	expression tag
Lys -6	-	expression tag
Thr -5	-	expression tag
Ile -4	-	expression tag
Ile -3	-	expression tag
Ala -2	-	expression tag
Leu -1	-	expression tag
Ser 0	-	expression tag
Tyr 1	-	expression tag
Ile 2	-	expression tag
Phe 3	-	expression tag
Cys 4	-	expression tag
Leu 5	-	expression tag
Val 6	-	expression tag
Phe 7	-	expression tag
Ala 8	-	expression tag
Asp 9	-	expression tag
Tyr 10	-	expression tag
Lys 11	-	expression tag
Asp 12	-	expression tag
Asp 13	-	expression tag
Asp 14	-	expression tag
Asp 15	-	expression tag
Leu 16	-	expression tag
Glu 17	-	expression tag
Val 18	-	expression tag
Leu 19	-	expression tag
Phe 20	-	expression tag
Gln 21	-	expression tag
Gly 22	-	expression tag
Pro 23	-	expression tag
Ala 24	-	expression tag
Leu 447	Pro 447	conflict
Pro 475	-	expression tag
Ala 476	-	expression tag
Gly 477	-	expression tag
Leu 478	-	expression tag
Glu 479	-	expression tag
Val 480	-	expression tag
Leu 481	-	expression tag
Phe 482	-	expression tag
Gln 483	-	expression tag
Gly 484	-	expression tag
Pro 485	-	expression tag
His 486	-	expression tag
His 487	-	expression tag
His 488	-	expression tag
His 489	-	expression tag
His 490	-	expression tag
His 491	-	expression tag
His 492	-	expression tag
His 493	-	expression tag

A: 1 - 394 (UniProt: P63092)

PDB	External Database	Details
Asn 54	Ser 54	conflict
Ala 226	Gly 226	conflict
Ala 268	Glu 268	conflict
Lys 271	Asn 271	conflict
Asp 274	Lys 274	conflict
Lys 280	Arg 280	conflict
Asp 284	Thr 284	conflict
Thr 285	Ile 285	conflict
Ser 366	Ala 366	engineered mutation

B: 2 - 340 (UniProt: P62873)

PDB	External Database	Details
Met -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
Ser 0	-	expression tag
Gly 1	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	7
	Total formula weight	186981.2
Non-Polymers*	Number of molecules	8
	Total formula weight	2476.8
All*	Total formula weight	189458.0

*Water molecules are not included.