7SPA

Chlorella virus Hyaluronan Synthase in the GlcNAc-primed, channel-open state

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	B	Nanobody 872	polymer	134	14783.2	1		Lama glama
2	C	Nanobody 881	polymer	137	15265.8	1		Lama glama
3	A	Hyaluronan synthase	polymer	570	65336.5	1	UniProt (M1H2Q1) Pfam (PF13641) In PDB	Paramecium bursaria Chlorella virus CZ-2
4	A	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	1	Chemie (NAG)
5	A	CHOLESTEROL HEMISUCCINATE	non-polymer		486.7	1	Chemie (Y01)
6	A	1,2-Distearoyl-sn-glycerophosphoethanolamine	non-polymer		748.1	1	Chemie (3PE)

Sequence modifications

A: 2 - 561 (UniProt: M1H2Q1)

PDB	External Database	Details
Met 0	-	initiating methionine
Gly 1	-	expression tag
Asn 302	Asp 302	engineered mutation
Leu 562	-	expression tag
Glu 563	-	expression tag
His 564	-	expression tag
His 565	-	expression tag
His 566	-	expression tag
His 567	-	expression tag
His 568	-	expression tag
His 569	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	95385.5
Non-Polymers*	Number of molecules	3
	Total formula weight	1456.0
All*	Total formula weight	96841.5

*Water molecules are not included.