7RXC

CryoEM structure of KDELR with Legobody

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	H	Fab_8D3_2 heavy chain	polymer	234	25252.2	1		Mus musculus (mouse)
2	L	Fab_8D3_2 light chain	polymer	219	24095.9	1		Mus musculus (mouse)
3	N	Nb_KR	polymer	129	14698.3	1		Vicugna pacos (Alpaca Lama pacos)
4	B	Maltodextrin-binding protein,Immunoglobulin G-binding protein A,Immunoglobulin G-binding protein G	polymer	545	59233.2	1	UniProt (A0A4Z0THX4) UniProt (P99134) UniProt (P06654) Pfam (PF02216) Pfam (PF01378) In PDB	Escherichia coli	IgG-binding protein A,Staphylococcal protein A,SpA,IgG-binding protein G
5	K	ER lumen protein-retaining receptor 2	polymer	264	30084.0	1	UniProt (Q5ZKX9) Pfam (PF00810) In PDB	Gallus gallus (Chicken)	KDEL endoplasmic reticulum protein retention receptor 2,KDEL receptor 2
6	A	alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose	branched		342.3	1	In PDB BIRD (PRD_900001) GlyTouCan (G07411ON)
7	K	(2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate	non-polymer		760.1	4	Chemie (POV)

Sequence modifications

B: 2 - 359 (UniProt: A0A4Z0THX4)

PDB	External Database	Details
Met 1	-	initiating methionine

B: 360 - 467 (UniProt: P99134)

PDB	External Database	Details
Ala 361	Gln 44	conflict
Leu 362	Asn 45	conflict
Ala 365	Tyr 48	conflict
Ile 367	Val 50	conflict
Ile 369	Asn 52	conflict
Thr 374	Asn 57	conflict
Glu 375	Ala 58	conflict
Glu 376	Asp 59	conflict
Val 391	Gln 74	conflict
Lys 393	Ala 76	conflict
Glu 394	Asn 77	conflict
Ile 395	Val 78	conflict
Ala 397	Gly 80	conflict
Lys 400	Gln 83	conflict
Glu 404	Asp 87	conflict
His 405	Ser 88	conflict
-	Ala 93	deletion
Gly 410	Asp 94	conflict
Gly 411	Ala 95	conflict
Ser 412	Gln 96	conflict
Gly 413	Gln 97	conflict
Gly 414	Asn 98	conflict
Ala 415	Asn 99	conflict
Gly 416	Phe 100	conflict
Ser 417	Asn 101	conflict
Gly 418	Lys 102	conflict
Gly 468	-	linker
Gly 469	-	linker
Gly 470	-	linker
Ser 471	-	linker
Gly 472	-	linker
Gly 473	-	linker
Gly 474	-	linker
Ser 475	-	linker
Gly 476	-	linker
Gly 477	-	linker
Ser 478	-	linker

B: 479 - 536 (UniProt: P06654)

PDB	External Database	Details
Gly 537	-	expression tag
Ser 538	-	expression tag
Gly 539	-	expression tag
His 540	-	expression tag
His 541	-	expression tag
His 542	-	expression tag
His 543	-	expression tag
His 544	-	expression tag
His 545	-	expression tag

K: 1 - 212 (UniProt: Q5ZKX9)

PDB	External Database	Details
Gly 213	-	expression tag
Ser 214	-	expression tag
Gly 215	-	expression tag
Gly 216	-	expression tag
Glu 217	-	expression tag
Asn 218	-	expression tag
Leu 219	-	expression tag
Tyr 220	-	expression tag
Phe 221	-	expression tag
Gln 222	-	expression tag
Ser 223	-	expression tag
Gly 224	-	expression tag
Gly 225	-	expression tag
Gly 226	-	expression tag
Met 227	-	expression tag
Asp 228	-	expression tag
Glu 229	-	expression tag
Lys 230	-	expression tag
Thr 231	-	expression tag
Thr 232	-	expression tag
Gly 233	-	expression tag
Trp 234	-	expression tag
Arg 235	-	expression tag
Gly 236	-	expression tag
Gly 237	-	expression tag
His 238	-	expression tag
Val 239	-	expression tag
Val 240	-	expression tag
Glu 241	-	expression tag
Gly 242	-	expression tag
Leu 243	-	expression tag
Ala 244	-	expression tag
Gly 245	-	expression tag
Glu 246	-	expression tag
Leu 247	-	expression tag
Glu 248	-	expression tag
Gln 249	-	expression tag
Leu 250	-	expression tag
Arg 251	-	expression tag
Ala 252	-	expression tag
Arg 253	-	expression tag
Leu 254	-	expression tag
Glu 255	-	expression tag
His 256	-	expression tag
His 257	-	expression tag
Pro 258	-	expression tag
Gln 259	-	expression tag
Gly 260	-	expression tag
Gln 261	-	expression tag
Arg 262	-	expression tag
Glu 263	-	expression tag
Pro 264	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	153363.5
Branched	Number of molecules	1
	Total formula weight	342.3
Non-Polymers*	Number of molecules	4
	Total formula weight	3040.3
All*	Total formula weight	156746.1

*Water molecules are not included.