7RMI

SP6-11 biased agonist bound to active human neurokinin 1 receptor in complex with miniGs/q70

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Guanine nucleotide-binding protein G(s) subunit alpha isoforms short, with certain residues mutated to match Guanine nucleotide-binding protein G(q) subunit	polymer	229	26451.0	1	UniProt (P63092) Pfam (PF00503) In PDB	Homo sapiens (Human)	miniGs/q70,Adenylate cyclase-stimulating G alpha protein
2	R	Substance-P receptor	polymer	418	47542.9	1	UniProt (P25103) Pfam (PF00001) In PDB	Homo sapiens (Human)	SPR,NK-1 receptor,NK-1R,Tachykinin receptor 1
3	S	Substance P 6-11	polymer	7	739.9	1		synthetic construct
4	B	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	370	40786.6	1	UniProt (P62873) Pfam (PF00400) In PDB	Homo sapiens (Human)	Transducin beta chain 1
5	G	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	68	7563.8	1	UniProt (P59768) Pfam (PF00631) In PDB	Homo sapiens (Human)	G gamma-I
6	N	Nanobody 35	polymer	142	15398.1	1		Lama glama

Sequence modifications

A: 26 - 195 (UniProt: P63092)

PDB	External Database	Details
Gly 25	-	expression tag
Asp 49	Gly 49	engineered mutation
Asn 50	Glu 50	engineered mutation

A: 204 - 394 (UniProt: P63092)

PDB	External Database	Details
Gly 196	-	linker
Gly 197	-	linker
Ser 198	-	linker
Gly 199	-	linker
Gly 200	-	linker
Ser 201	-	linker
Gly 202	-	linker
Gly 203	-	linker
Asp 249	Ala 249	engineered mutation
Asp 252	Ser 252	engineered mutation
-	Asn 254	deletion
-	Met 255	deletion
-	Val 256	deletion
-	Ile 257	deletion
-	Arg 258	deletion
-	Glu 259	deletion
-	Asp 260	deletion
-	Asn 261	deletion
-	Gln 262	deletion
-	Thr 263	deletion
Asp 272	Leu 272	engineered mutation
Ala 372	Ile 372	engineered mutation
Ile 375	Val 375	engineered mutation
Lys 380	Arg 380	engineered mutation
Leu 384	Gln 384	engineered mutation
Gln 385	Arg 385	engineered mutation
Asn 387	His 387	engineered mutation
Glu 390	Gln 390	engineered mutation
Asn 392	Glu 392	engineered mutation
Val 394	Leu 394	engineered mutation

R: 1 - 407 (UniProt: P25103)

PDB	External Database	Details
Asp -10	-	expression tag
Tyr -9	-	expression tag
Lys -8	-	expression tag
Asp -7	-	expression tag
Asp -6	-	expression tag
Asp -5	-	expression tag
Asp -4	-	expression tag
Ala -3	-	expression tag
Ser -2	-	expression tag
Ile -1	-	expression tag
Asp 0	-	expression tag

B: 2 - 340 (UniProt: P62873)

PDB	External Database	Details
Met -29	-	initiating methionine
His -28	-	expression tag
His -27	-	expression tag
His -26	-	expression tag
His -25	-	expression tag
His -24	-	expression tag
His -23	-	expression tag
Leu -22	-	expression tag
Glu -21	-	expression tag
Val -20	-	expression tag
Leu -19	-	expression tag
Phe -18	-	expression tag
Gln -17	-	expression tag
Gly -16	-	expression tag
Pro -15	-	expression tag
Glu -14	-	expression tag
Asp -13	-	expression tag
Gln -12	-	expression tag
Val -11	-	expression tag
Asp -10	-	expression tag
Pro -9	-	expression tag
Arg -8	-	expression tag
Leu -7	-	expression tag
Ile -6	-	expression tag
Asp -5	-	expression tag
Gly -4	-	expression tag
Lys -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
Ser 0	-	expression tag
Gly 1	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	138482.1
All*	Total formula weight	138482.1

*Water molecules are not included.