7R4H

phospho-STING binding to adaptor protein complex-1

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	B	AP-1 complex subunit beta-1	polymer	584	66008.4	1	UniProt (Q10567) Pfam (PF01602) In PDB	Homo sapiens (human)	Adaptor protein complex AP-1 subunit beta-1,Adaptor-related protein complex 1 subunit beta-1,Beta-1-adaptin,Beta-adaptin 1,Clathrin assembly protein complex 1 beta large chain,Golgi adaptor HA1/AP1 adaptin beta subunit
2	C, H	ADP-ribosylation factor 1	polymer	165	18936.6	2	UniProt (P84077) Pfam (PF00025) In PDB	Homo sapiens (human)
3	G	AP-1 complex subunit gamma-1	polymer	595	67399.2	1	UniProt (P22892) Pfam (PF01602) In PDB	Mus musculus (house mouse)	Adaptor protein complex AP-1 subunit gamma-1,Adaptor-related protein complex 1 subunit gamma-1,Clathrin assembly protein complex 1 gamma-1 large chain,Gamma-adaptin,Gamma1-adaptin,Golgi adaptor HA1/AP1 adaptin subunit gamma-1
4	L	Stimulator of interferon genes protein	polymer	9	1065.1	1	UniProt (Q86WV6) In PDB	Homo sapiens (human)	hSTING,Endoplasmic reticulum interferon stimulator,ERIS,Mediator of IRF3 activation,hMITA,Transmembrane protein 173
5	M	AP-1 complex subunit mu-1	polymer	423	48606.7	1	UniProt (P35585) Pfam (PF01217) Pfam (PF00928) In PDB	Mus musculus (house mouse)	AP-mu chain family member mu1A,Adaptor protein complex AP-1 subunit mu-1,Adaptor-related protein complex 1 subunit mu-1,Clathrin assembly protein complex 1 mu-1 medium chain 1,Clathrin coat assembly protein AP47,Clathrin coat-associated protein AP47,Golgi adaptor HA1/AP1 adaptin mu-1 subunit,Mu-adaptin 1,Mu1A-adaptin
6	S	AP-1 complex subunit sigma-3	polymer	154	18321.3	1	UniProt (Q96PC3) Pfam (PF01217) In PDB	Homo sapiens (human)	Adaptor protein complex AP-1 subunit sigma-1C,Adaptor-related protein complex 1 subunit sigma-1C,Clathrin assembly protein complex 1 sigma-1C small chain,Golgi adaptor HA1/AP1 adaptin sigma-1C subunit,Sigma 1C subunit of AP-1 clathrin,Sigma-adaptin 1C,Sigma1C-adaptin
7	C, H	GUANOSINE-5'-TRIPHOSPHATE	non-polymer		523.2	2	Chemie (GTP)
8	C, H	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)

Sequence modifications

B: 1 - 584 (UniProt: Q10567)

PDB	External Database	Details
Arg 359	Lys 359	engineered mutation
Lys 476	Glu 476	engineered mutation

C, H: 17 - 181 (UniProt: P84077)

PDB	External Database	Details
Leu 71	Gln 71	engineered mutation

S: 1 - 154 (UniProt: Q96PC3)

PDB	External Database	Details
Cys 148	Ser 148	conflict

Contents of the asymmetric unit

Polymers	Number of chains	7
	Total formula weight	239274.0
Non-Polymers*	Number of molecules	4
	Total formula weight	1095.0
All*	Total formula weight	240369.0

*Water molecules are not included.