7PLO

H. sapiens replisome-CUL2/LRR1 complex

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	2	DNA replication licensing factor MCM2	polymer	904	102034.1	1	UniProt (P49736) Pfam (PF12619) Pfam (PF14551) Pfam (PF17207) Pfam (PF00493) Pfam (PF17855) In PDB	Homo sapiens (human)	Minichromosome maintenance protein 2 homolog,Nuclear protein BM28
2	3	DNA replication licensing factor MCM3	polymer	808	91110.9	1	UniProt (P25205) Pfam (PF14551) Pfam (PF17207) Pfam (PF00493) Pfam (PF17855) In PDB	Homo sapiens (human)	DNA polymerase alpha holoenzyme-associated protein P1,P1-MCM3,RLF subunit beta,p102
3	4	DNA replication licensing factor MCM4	polymer	863	96684.9	1	UniProt (P33991) Pfam (PF14551) Pfam (PF17207) Pfam (PF00493) Pfam (PF17855) Pfam (PF21128) In PDB	Homo sapiens (human)	CDC21 homolog,P1-CDC21
4	5	DNA replication licensing factor MCM5	polymer	734	82406.6	1	UniProt (P33992) Pfam (PF14551) Pfam (PF17207) Pfam (PF00493) Pfam (PF17855) In PDB	Homo sapiens (human)	CDC46 homolog,P1-CDC46
5	6	DNA replication licensing factor MCM6	polymer	821	93010.3	1	UniProt (Q14566) Pfam (PF14551) Pfam (PF17207) Pfam (PF00493) Pfam (PF17855) Pfam (PF18263) In PDB	Homo sapiens (human)	p105MCM
6	7	DNA replication licensing factor MCM7	polymer	719	81411.9	1	UniProt (P33993) Pfam (PF14551) Pfam (PF17207) Pfam (PF00493) Pfam (PF17855) In PDB	Homo sapiens (human)	CDC47 homolog,P1.1-MCM3
7	A	DNA polymerase epsilon subunit 2	polymer	527	59600.9	1	UniProt (P56282) Pfam (PF12213) Pfam (PF04042) In PDB	Homo sapiens (human)	DNA polymerase II subunit 2,DNA polymerase epsilon subunit B
8	B	DNA polymerase epsilon catalytic subunit A	polymer	2286	261855.3	1	UniProt (Q07864) Pfam (PF03104) Pfam (PF00136) Pfam (PF08490) In PDB	Homo sapiens (human)	3'-5' exodeoxyribonuclease,DNA polymerase II subunit A
9	C	Cell division control protein 45 homolog	polymer	569	66016.9	1	UniProt (O75419) Pfam (PF02724) In PDB	Homo sapiens (human)	PORC-PI-1
10	D	DNA replication complex GINS protein PSF1	polymer	196	23022.5	1	UniProt (Q14691) Pfam (PF05916) In PDB	Homo sapiens (human)	GINS complex subunit 1
11	E	DNA replication complex GINS protein PSF2	polymer	185	21453.7	1	UniProt (Q9Y248) Pfam (PF05916) In PDB	Homo sapiens (human)	GINS complex subunit 2
12	F	DNA replication complex GINS protein PSF3	polymer	216	24562.6	1	UniProt (Q9BRX5) Pfam (PF05916) In PDB	Homo sapiens (human)	GINS complex subunit 3
13	G	DNA replication complex GINS protein SLD5	polymer	262	30114.2	1	UniProt (Q9BRT9) Pfam (PF05916) Pfam (PF16922) In PDB	Homo sapiens (human)	GINS complex subunit 4
14	H, I, J	WD repeat and HMG-box DNA-binding protein 1	polymer	1161	130098.1	3	UniProt (O75717) Pfam (PF00400) Pfam (PF12894) Pfam (PF12341) Pfam (PF20946) In PDB	Homo sapiens (human)	Acidic nucleoplasmic DNA-binding protein 1,And-1
15	K	Protein timeless homolog	polymer	1209	138903.0	1	UniProt (Q9UNS1) Pfam (PF04821) Pfam (PF05029) In PDB	Homo sapiens (human)	hTIM
16	L	TIMELESS-interacting protein	polymer	301	34600.2	1	UniProt (Q9BVW5) Pfam (PF07962) In PDB	Homo sapiens (human)
17	M	Leading strand DNA	polymer	85	26396.8	1		Homo sapiens
18	N	Lagging strand DNA	polymer	41	12279.9	1		Homo sapiens
19	O	Leucine-rich repeat protein 1	polymer	414	46789.6	1	UniProt (Q96L50) Pfam (PF12799) Pfam (PF00560) In PDB	Homo sapiens (human)	4-1BB-mediated-signaling molecule,4-1BBlrr,LRR-repeat protein 1,LRR-1,Peptidylprolyl isomerase-like 5
20	P	Elongin-B	polymer	118	13147.8	1	UniProt (Q15370) Pfam (PF00240) In PDB	Homo sapiens (human)	EloB,Elongin 18 kDa subunit,RNA polymerase II transcription factor SIII subunit B,SIII p18,Transcription elongation factor B polypeptide 2
21	Q	Claspin	polymer	1371	155184.7	1	UniProt (Q9HAW4) In PDB	Homo sapiens (human)	hClaspin
22	R	Elongin-C	polymer	112	12485.1	1	UniProt (Q15369) Pfam (PF03931) In PDB	Homo sapiens (human)	EloC,Elongin 15 kDa subunit,RNA polymerase II transcription factor SIII subunit C,SIII p15,Transcription elongation factor B polypeptide 1
23	S	Cullin-2	polymer	745	87098.9	1	UniProt (Q13617) Pfam (PF00888) Pfam (PF10557) In PDB	Homo sapiens (human)	CUL-2
24	T	E3 ubiquitin-protein ligase RBX1	polymer	108	12290.0	1	UniProt (P62877) Pfam (PF12678) In PDB	Homo sapiens (human)	E3 ubiquitin-protein transferase RBX1,Protein ZYP,RING finger protein 75,RING-box protein 1,Rbx1,Regulator of cullins 1,ROC1
25	2, 4, 5, 6, 7...	ZINC ION	non-polymer		65.4	8	Chemie (ZN)
26	2, 3, 5	MAGNESIUM ION	non-polymer		24.3	3	Chemie (MG)
27	2, 3, 5	PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER	non-polymer		506.2	3	Chemie (ANP)
28	A	SULFATE ION	non-polymer		96.1	1	Chemie (SO4)

Sequence modifications

C: 1 - 135 (UniProt: O75419)
C: 136 - 566 (UniProt: O75419)

PDB	External Database	Details
Asp 135	-	linker
Tyr 136	-	linker
Lys 136	-	linker
Asp 136	-	linker
Asp 136	-	linker
Asp 136	-	linker

G: 1 - 223 (UniProt: Q9BRT9)

PDB	External Database	Details
Met -38	-	initiating methionine
Trp -37	-	expression tag
Ser -36	-	expression tag
His -35	-	expression tag
Pro -34	-	expression tag
Gln -33	-	expression tag
Phe -32	-	expression tag
Glu -31	-	expression tag
Lys -30	-	expression tag
Gly -29	-	expression tag
Gly -28	-	expression tag
Gly -27	-	expression tag
Ser -26	-	expression tag
Gly -25	-	expression tag
Gly -24	-	expression tag
Gly -23	-	expression tag
Ser -22	-	expression tag
Gly -21	-	expression tag
Gly -20	-	expression tag
Ser -19	-	expression tag
Ala -18	-	expression tag
Trp -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
Pro -14	-	expression tag
Gln -13	-	expression tag
Phe -12	-	expression tag
Glu -11	-	expression tag
Lys -10	-	expression tag
Ser -9	-	expression tag
Gly -8	-	expression tag
Leu -7	-	expression tag
Glu -6	-	expression tag
Val -5	-	expression tag
Leu -4	-	expression tag
Phe -3	-	expression tag
Gln -2	-	expression tag
Gly -1	-	expression tag
Pro 0	-	expression tag

H, I, J: 1 - 1129 (UniProt: O75717)

PDB	External Database	Details
Met -31	-	initiating methionine
Asp -30	-	expression tag
Tyr -29	-	expression tag
Lys -28	-	expression tag
Asp -27	-	expression tag
Asp -26	-	expression tag
Asp -25	-	expression tag
Asp -24	-	expression tag
Lys -23	-	expression tag
Asp -22	-	expression tag
Tyr -21	-	expression tag
Lys -20	-	expression tag
Asp -19	-	expression tag
Asp -18	-	expression tag
Asp -17	-	expression tag
Asp -16	-	expression tag
Lys -15	-	expression tag
Asp -14	-	expression tag
Tyr -13	-	expression tag
Lys -12	-	expression tag
Asp -11	-	expression tag
Asp -10	-	expression tag
Asp -9	-	expression tag
Asp -8	-	expression tag
Lys -7	-	expression tag
Glu -6	-	expression tag
Asn -5	-	expression tag
Leu -4	-	expression tag
Tyr -3	-	expression tag
Phe -2	-	expression tag
Gln -1	-	expression tag
Gly 0	-	expression tag

K: 1 - 1208 (UniProt: Q9UNS1)

PDB	External Database	Details
Gly 0	-	expression tag

Q: 1 - 1339 (UniProt: Q9HAW4)

PDB	External Database	Details
Leu 1340	-	expression tag
Glu 1341	-	expression tag
Val 1342	-	expression tag
Leu 1343	-	expression tag
Phe 1344	-	expression tag
Gln 1345	-	expression tag
Gly 1346	-	expression tag
Pro 1347	-	expression tag
Asp 1348	-	expression tag
Tyr 1349	-	expression tag
Lys 1350	-	expression tag
Asp 1351	-	expression tag
Asp 1352	-	expression tag
Asp 1353	-	expression tag
Asp 1354	-	expression tag
Lys 1355	-	expression tag
Asp 1356	-	expression tag
Tyr 1357	-	expression tag
Lys 1358	-	expression tag
Asp 1359	-	expression tag
Asp 1360	-	expression tag
Asp 1361	-	expression tag
Asp 1362	-	expression tag
Lys 1363	-	expression tag
Asp 1364	-	expression tag
Tyr 1365	-	expression tag
Lys 1366	-	expression tag
Asp 1367	-	expression tag
Asp 1368	-	expression tag
Asp 1369	-	expression tag
Asp 1370	-	expression tag
Lys 1371	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	26
	Total formula weight	1962755.3
Non-Polymers*	Number of molecules	15
	Total formula weight	2210.8
All*	Total formula weight	1964966.1

*Water molecules are not included.