7PFE

Nucleosome 2 of the 4x197 nucleosome array containing H1

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	a, e	Histone H3.2	polymer	136	15389.0	2	UniProt (Q71DI3) Pfam (PF00125) In PDB	Homo sapiens (Human)	Histone H3/m,Histone H3/o
2	b, f	Histone H4	polymer	103	11394.4	2	UniProt (P62805) Pfam (PF15511) In PDB	Homo sapiens (Human)
3	c, g	Histone H2A type 1-B/E	polymer	147	16344.9	2	UniProt (P04908) Pfam (PF00125) Pfam (PF16211) In PDB	Homo sapiens (Human)	Histone H2A.2,Histone H2A/a,Histone H2A/m
4	d, h	Histone H2B type 1-K	polymer	126	13921.2	2	UniProt (O60814) Pfam (PF00125) In PDB	Homo sapiens (Human)	H2B K,HIRA-interacting protein 1
5	J	DNA (177-MER)	polymer	177	54862.9	1		synthetic construct
6	I	DNA (177-MER)	polymer	177	54422.7	1		synthetic construct
7	u	Histone H1.4	polymer	218	21800.3	1	UniProt (P10412) Pfam (PF00538) In PDB	Homo sapiens (Human)	Histone H1b,Histone H1s-4

Sequence modifications

a, e: 0 - 135 (UniProt: Q71DI3)

PDB	External Database	Details
Ala 110	Cys 111	engineered mutation

c, g: 0 - 129 (UniProt: P04908)

PDB	External Database	Details
His -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
Glu -11	-	expression tag
Asn -10	-	expression tag
Leu -9	-	expression tag
Tyr -8	-	expression tag
Phe -7	-	expression tag
Gln -6	-	expression tag
Ser -5	-	expression tag
Asn -4	-	expression tag
Ala -3	-	expression tag
Pro -2	-	expression tag
Trp -1	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	11
	Total formula weight	245185.1
All*	Total formula weight	245185.1

*Water molecules are not included.