7NCX

Crystal structure of GH30 (double mutant EE) from Thermothelomyces thermophila.

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	AAA	GH30 family xylanase	polymer	482	51728.9	1	UniProt (G2Q1N4) Pfam (PF14587) Pfam (PF17189) In PDB	Myceliophthora thermophila (strain ATCC 42464 / BCRC 31852 / DSM 1799)
2	BBB	alpha-D-mannopyranose-(1-3)-alpha-D-mannopyranose-(1-6)-[alpha-D-mannopyranose-(1-3)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		1073.0	1	In PDB GlyTouCan (G09724ZC)
3	AAA	GLYCEROL	non-polymer		92.1	1	Chemie (GOL)
4	AAA	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	1	Chemie (PEG)
5	AAA	1,2-ETHANEDIOL	non-polymer		62.1	2	Chemie (EDO)
6	AAA	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	1	Chemie (NAG)
7		water	water		18.0	515	Chemie (HOH)

Sequence modifications

AAA: 20 - 477 (UniProt: G2Q1N4)

PDB	External Database	Details
Ser 18	-	expression tag
Met 19	-	expression tag
Ala 205	Glu 205	engineered mutation
Ala 295	Glu 295	engineered mutation
Leu 478	-	expression tag
Glu 479	-	expression tag
Gln 480	-	expression tag
Lys 481	-	expression tag
Leu 482	-	expression tag
Ile 483	-	expression tag
Ser 484	-	expression tag
Glu 485	-	expression tag
Glu 486	-	expression tag
Asp 487	-	expression tag
Leu 488	-	expression tag
Asn 489	-	expression tag
Ser 490	-	expression tag
Ala 491	-	expression tag
Val 492	-	expression tag
Asp 493	-	expression tag
His 494	-	expression tag
His 495	-	expression tag
His 496	-	expression tag
His 497	-	expression tag
His 498	-	expression tag
His 499	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	51728.9
Branched	Number of molecules	1
	Total formula weight	1073.0
Non-Polymers*	Number of molecules	5
	Total formula weight	543.6
All*	Total formula weight	53345.5

*Water molecules are not included.