7MFG

Cryo-EM structure of the VRC310 clinical trial, vaccine-elicited, human antibody 310-030-1D06 Fab in complex with an H1 NC99 HA trimer

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C, G	Hemagglutinin HA1 chain	polymer	326	36407.9	3	UniProt (Q6WG00) Pfam (PF00509) In PDB	Influenza A virus
2	B, D, I	Hemagglutinin HA2 chain	polymer	222	25263.1	3	UniProt (Q289M7) Pfam (PF00509) UniProt (by SIFTS) (Q6WG00) In PDB	Influenza A virus
3	H, E, J	310-030-1D06 Heavy	polymer	120	13000.5	3		Homo sapiens (Human)
4	L, F, K	310-030-1D06 Light	polymer	108	11658.9	3		Homo sapiens (Human)
5	M, N, O	beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		586.5	3	In PDB GlyTouCan (G15407YE)
6	C, G, A	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	21	Chemie (NAG)

Sequence modifications

A, C, G: 11 - 329 (UniProt: Q6WG00)

PDB	External Database	Details
Cys 30	Leu 37	conflict

B, D, I: 1 - 176 (UniProt: Q289M7)

PDB	External Database	Details
Cys 47	Gly 390	conflict
Ser 177	-	expression tag
Gly 178	-	expression tag
Arg 179	-	expression tag
Leu 180	-	expression tag
Val 181	-	expression tag
Pro 182	-	expression tag
Arg 183	-	expression tag
Gly 184	-	expression tag
Ser 185	-	expression tag
Pro 186	-	expression tag
Gly 187	-	expression tag
Ser 188	-	expression tag
Gly 189	-	expression tag
Tyr 190	-	expression tag
Ile 191	-	expression tag
Pro 192	-	expression tag
Glu 193	-	expression tag
Ala 194	-	expression tag
Pro 195	-	expression tag
Arg 196	-	expression tag
Asp 197	-	expression tag
Gly 198	-	expression tag
Gln 199	-	expression tag
Ala 200	-	expression tag
Tyr 201	-	expression tag
Val 202	-	expression tag
Arg 203	-	expression tag
Lys 204	-	expression tag
Asp 205	-	expression tag
Gly 206	-	expression tag
Glu 207	-	expression tag
Trp 208	-	expression tag
Val 209	-	expression tag
Leu 210	-	expression tag
Leu 211	-	expression tag
Ser 212	-	expression tag
Thr 213	-	expression tag
Phe 214	-	expression tag
Leu 215	-	expression tag
Gly 216	-	expression tag
His 217	-	expression tag
His 218	-	expression tag
His 219	-	expression tag
His 220	-	expression tag
His 221	-	expression tag
His 222	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	12
	Total formula weight	258991.0
Branched	Number of molecules	3
	Total formula weight	1759.6
Non-Polymers*	Number of molecules	21
	Total formula weight	4645.4
All*	Total formula weight	265395.9

*Water molecules are not included.