7L9E

Crystal structure of apo-alpha glucosidase

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	E, G	Neutral alpha-glucosidase AB Trypsin-cleaved Fragment #1	polymer	184	20475.9	2	UniProt (Q8BHN3) Pfam (PF13802) In PDB	Mus musculus (Mouse)	Alpha-glucosidase 2,Glucosidase II subunit alpha
2	F, H	Neutral alpha-glucosidase AB Trypsin-cleaved Fragment #2	polymer	107	12052.3	2	UniProt (Q8BHN3) Pfam (PF13802) In PDB	Mus musculus (Mouse)	Alpha-glucosidase 2,Glucosidase II subunit alpha
3	A, C	Neutral alpha-glucosidase AB Trypsin-cleaved Fragment #3	polymer	609	69898.7	2	UniProt (Q8BHN3) Pfam (PF01055) Pfam (PF21365) In PDB	Mus musculus (Mouse)	Alpha-glucosidase 2,Glucosidase II subunit alpha
4	B, D	Glucosidase 2 subunit beta	polymer	134	14323.0	2	UniProt (O08795) Pfam (PF12999) In PDB	Mus musculus (Mouse)	80K-H protein,Glucosidase II subunit beta,Protein kinase C substrate 60.1 kDa protein heavy chain,PKCSH
5	B, G, C, E, F...	1,2-ETHANEDIOL	non-polymer		62.1	23	Chemie (EDO)
6	A	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	3	Chemie (PEG)
7	A	TETRAETHYLENE GLYCOL	non-polymer		194.2	1	Chemie (PG4)
8	A, C	SULFATE ION	non-polymer		96.1	3	Chemie (SO4)
9	B, D	CALCIUM ION	non-polymer		40.1	4	Chemie (CA)
10		water	water		18.0	680	Chemie (HOH)

Sequence modifications

E, G: 33 - 185 (UniProt: Q8BHN3)

PDB	External Database	Details
Met 2	-	initiating methionine
Gly 3	-	expression tag
Ile 4	-	expression tag
Leu 5	-	expression tag
Pro 6	-	expression tag
Ser 7	-	expression tag
Pro 8	-	expression tag
Gly 9	-	expression tag
Met 10	-	expression tag
Pro 11	-	expression tag
Ala 12	-	expression tag
Leu 13	-	expression tag
Leu 14	-	expression tag
Ser 15	-	expression tag
Leu 16	-	expression tag
Val 17	-	expression tag
Ser 18	-	expression tag
Leu 19	-	expression tag
Leu 20	-	expression tag
Ser 21	-	expression tag
Val 22	-	expression tag
Leu 23	-	expression tag
Leu 24	-	expression tag
Met 25	-	expression tag
Gly 26	-	expression tag
Cys 27	-	expression tag
Val 28	-	expression tag
Ala 29	-	expression tag
Glu 30	-	expression tag
Thr 31	-	expression tag
Gly 32	-	expression tag
Asp 97	Asn 97	engineered mutation

A, C: 370 - 966 (UniProt: Q8BHN3)

PDB	External Database	Details
Ser 967	-	expression tag
Ala 968	-	expression tag
Trp 969	-	expression tag
Ser 970	-	expression tag
His 971	-	expression tag
Pro 972	-	expression tag
Gln 973	-	expression tag
Phe 974	-	expression tag
Glu 975	-	expression tag
Lys 976	-	expression tag
Leu 977	-	expression tag
Glu 978	-	expression tag

B, D: 15 - 117 (UniProt: O08795)

PDB	External Database	Details
Met -16	-	initiating methionine
Gly -15	-	expression tag
Ile -14	-	expression tag
Leu -13	-	expression tag
Pro -12	-	expression tag
Ser -11	-	expression tag
Pro -10	-	expression tag
Gly -9	-	expression tag
Met -8	-	expression tag
Pro -7	-	expression tag
Ala -6	-	expression tag
Leu -5	-	expression tag
Leu -4	-	expression tag
Ser -3	-	expression tag
Leu -2	-	expression tag
Val -1	-	expression tag
Ser 0	-	expression tag
Leu 1	-	expression tag
Leu 2	-	expression tag
Ser 3	-	expression tag
Val 4	-	expression tag
Leu 5	-	expression tag
Leu 6	-	expression tag
Met 7	-	expression tag
Gly 8	-	expression tag
Cys 9	-	expression tag
Val 10	-	expression tag
Ala 11	-	expression tag
Glu 12	-	expression tag
Thr 13	-	expression tag
Gly 14	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	233499.9
Non-Polymers*	Number of molecules	34
	Total formula weight	2388.7
All*	Total formula weight	235888.5

*Water molecules are not included.