7KAU

Cryo-EM structure of the Sec complex from S. cerevisiae, Sec61 pore ring and Sec63 FN3 double mutant, class with Sec62

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Protein transport protein SEC61	polymer	480	52936.1	1	UniProt (P32915) Pfam (PF10559) Pfam (PF00344) In PDB	Saccharomyces cerevisiae BY4741	Sec61 complex subunit SEC61,Sec61 complex subunit alpha
2	C	Protein transport protein SSS1	polymer	80	8958.6	1	UniProt (P35179) Pfam (PF00584) In PDB	Saccharomyces cerevisiae BY4741	Sec61 complex subunit SSS1,Sec61 complex subunit gamma,Ssh1 complex subunit SSS1,Ssh1 complex subunit gamma
3	B	Protein transport protein SBH1	polymer	82	8723.2	1	UniProt (P52870) Pfam (PF03911) In PDB	Saccharomyces cerevisiae BY4741	Sec61 complex subunit SBH1,Sec61 complex subunit beta
4	D	Protein translocation protein SEC63	polymer	676	76312.0	1	UniProt (P14906) Pfam (PF00226) In PDB	Saccharomyces cerevisiae BY4741	Protein NPL1,Sec62/63 complex 73 kDa subunit
5	E	Translocation protein SEC66	polymer	206	24263.9	1	UniProt (P33754) Pfam (PF09802) In PDB	Saccharomyces cerevisiae BY4741	Protein HSS1,Sec62/63 complex 31.5 kDa subunit
6	F	Translocation protein SEC72	polymer	193	21631.1	1	UniProt (P39742) In PDB	Saccharomyces cerevisiae BY4741	Sec62/63 complex 23 kDa subunit,p23
7	G	Protein transport protein Sec62	polymer	56	4783.9	1		Saccharomyces cerevisiae BY4741

Sequence modifications

A: 1 - 480 (UniProt: P32915)

PDB	External Database	Details
Leu 90	Met 90	variant
Ile 185	Thr 185	variant
Ile 294	Met 294	variant
Leu 450	Met 450	variant

D: 2 - 663 (UniProt: P14906)

PDB	External Database	Details
Gly -13	-	expression tag
Gly -12	-	expression tag
Ser -11	-	expression tag
Gly -10	-	expression tag
Gly -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Gly -6	-	expression tag
Ser -5	-	expression tag
Gly -4	-	expression tag
Gly -3	-	expression tag
Ser -2	-	expression tag
Gly -1	-	expression tag
Gly 0	-	expression tag
Ser 1	-	expression tag
-	Glu 440	deletion
-	Asn 441	deletion
-	Gln 442	deletion
-	Val 443	deletion
-	Thr 444	deletion
-	Pro 445	deletion
-	Ser 446	deletion
-	Ser 447	deletion
Arg 440	Thr 448	conflict
Ser 481	Phe 481	conflict
Gly 664	-	expression tag
Glu 665	-	expression tag
Asn 666	-	expression tag
Leu 667	-	expression tag
Tyr 668	-	expression tag
Phe 669	-	expression tag
Gln 670	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	7
	Total formula weight	197608.8
All*	Total formula weight	197608.8

*Water molecules are not included.