7JHG

Cryo-EM structure of ATP-bound fully inactive AMPK in complex with Dorsomorphin (Compound C) and Fab-nanobody

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	5'-AMP-activated protein kinase catalytic subunit alpha-1	polymer	484	56004.4	1	UniProt (Q13131) Pfam (PF00069) Pfam (PF21147) Pfam (PF16579) In PDB	Homo sapiens (Human)	AMPK subunit alpha-1,Acetyl-CoA carboxylase kinase,ACACA kinase,Hydroxymethylglutaryl-CoA reductase kinase,HMGCR kinase,Tau-protein kinase PRKAA1
2	B	5'-AMP-activated protein kinase subunit beta-2	polymer	198	22384.7	1	UniProt (O43741) Pfam (PF16561) Pfam (PF04739) In PDB	Homo sapiens (Human)	AMPK subunit beta-2
3	G	5'-AMP-activated protein kinase subunit gamma-1	polymer	306	34833.4	1	UniProt (P54619) Pfam (PF00571) In PDB	Homo sapiens (Human)	AMPKg
4	M	Maltodextrin-binding protein	polymer	373	40827.1	1	UniProt (C3SHQ8) Pfam (PF01547) UniProt (by SIFTS) (P0AEX9) In PDB	Escherichia coli K-12
5	L	Fab light chain	polymer	215	23212.7	1		synthetic construct
6	H	Fab heavy chain	polymer	237	25483.5	1		synthetic construct
7	N	Nanobody	polymer	159	17414.4	1		synthetic construct
8	D	alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose	branched		342.3	1	In PDB BIRD (PRD_900001) GlyTouCan (G07411ON)
9	A	6-[4-(2-piperidin-1-ylethoxy)phenyl]-3-pyridin-4-ylpyrazolo[1,5-a]pyrimidine	non-polymer		399.5	1	Chemie (TAK)
10	G	ADENOSINE-5'-TRIPHOSPHATE	non-polymer		507.2	1	Chemie (ATP)
11	G	ADENOSINE-5'-DIPHOSPHATE	non-polymer		427.2	1	Chemie (ADP)
12	G	ADENOSINE MONOPHOSPHATE	non-polymer		347.2	1	Chemie (AMP)

Sequence modifications

A: 13 - 471 (UniProt: Q13131)
A: 472 - 550 (UniProt: Q13131)
B: 75 - 272 (UniProt: O43741)

G: 24 - 327 (UniProt: P54619)

PDB	External Database	Details
Met 22	-	expression tag
Gly 23	-	expression tag

M: 0 - 366 (UniProt: C3SHQ8)

Sequence viewer

Contents of the asymmetric unit

*Water molecules are not included.