7EDC

Crystal structure of mutant tRNA [Gm18] methyltransferase TrmH (E107G) in complex with S-adenosyl-L-methionine from Escherichia coli

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	tRNA (guanosine(18)-2'-O)-methyltransferase	polymer	249	27472.4	1	UniProt (C3SM77) Pfam (PF00588) Pfam (PF12105) UniProt (by SIFTS) (P0AGJ2) In PDB	Escherichia coli	tRNA [Gm18] methyltransferase
2	A	S-ADENOSYLMETHIONINE	non-polymer		398.4	1	Chemie (SAM)
3	A	PHOSPHATE ION	non-polymer		95.0	1	Chemie (PO4)
4		water	water		18.0	66	Chemie (HOH)

Sequence modifications

A: 1 - 229 (UniProt: C3SM77)

PDB	External Database	Details
Met -19	-	initiating methionine
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
Gly 107	Glu 107	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	27472.4
Non-Polymers*	Number of molecules	2
	Total formula weight	493.4
All*	Total formula weight	27965.8

*Water molecules are not included.