7CRH

Cryo-EM structure of SKF83959 bound dopamine receptor DRD1-Gs signaling complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Guanine nucleotide-binding protein G(s) subunit alpha isoforms short	polymer	394	45769.6	1	UniProt (P63092) Pfam (PF00503) In PDB	Homo sapiens (Human)	Adenylate cyclase-stimulating G alpha protein
2	B	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	356	39141.8	1	UniProt (P62873) Pfam (PF00400) In PDB	Homo sapiens (Human)	Transducin beta chain 1
3	G	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7861.1	1	UniProt (P59768) Pfam (PF00631) In PDB	Homo sapiens (Human)	G gamma-I
4	N	Nanobody35	polymer	156	16926.1	1		Lama glama
5	R	D(1A) dopamine receptor	polymer	446	49339.2	1	UniProt (P21728) Pfam (PF00001) In PDB	Homo sapiens (Human)	Dopamine D1 receptor
6	R	(1S)-6-chloranyl-3-methyl-1-(3-methylphenyl)-1,2,4,5-tetrahydro-3-benzazepine-7,8-diol	non-polymer		317.8	1	Chemie (GBU)

Sequence modifications

A: 1 - 394 (UniProt: P63092)

PDB	External Database	Details
Thr 205	Ser 205	engineered mutation
Ala 226	Gly 226	engineered mutation
Ser 366	Ala 366	engineered mutation

B: 2 - 340 (UniProt: P62873)

PDB	External Database	Details
Met -15	-	initiating methionine
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
Leu -10	-	expression tag
Glu -9	-	expression tag
Val -8	-	expression tag
Leu -7	-	expression tag
Phe -6	-	expression tag
Gln -5	-	expression tag
Gly -4	-	expression tag
Pro -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
Ser 0	-	expression tag
Gly 1	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	159037.8
Non-Polymers*	Number of molecules	1
	Total formula weight	317.8
All*	Total formula weight	159355.6

*Water molecules are not included.