7C8Z

Crystal structure of salicylate 5-hydroxylase NagGH (a Rieske non-heme iron-dependent monooxgenase)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C, E, G	Salicylate 5-hydroxylase, large oxygenase component	polymer	442	50949.4	4	UniProt (O52379) Pfam (PF00355) Pfam (PF00848) In PDB	Ralstonia sp.	S5H large oxygenase component,Salicylate 5-hydroxylase,oxygenase component NagG,Salicylate 5-monooxygenase,hydroxylase large subunit
2	B, D, F, H	Salicylate 5-hydroxylase, small oxygenase component	polymer	161	18834.4	4	UniProt (O52380) Pfam (PF13577) In PDB	Ralstonia sp.	S5H small oxygenase component,Salicylate 5-hydroxylase,oxygenase component NagH,Salicylate 5-monooxygenase,hydroxylase small subunit
3	A, C, E, G	FE2/S2 (INORGANIC) CLUSTER	non-polymer		175.8	4	Chemie (FES)
4	A, C, E, G	FE (III) ION	non-polymer		55.8	4	Chemie (FE)
5		water	water		18.0	326	Chemie (HOH)

Sequence modifications

A, C, E, G: 1 - 423 (UniProt: O52379)

PDB	External Database	Details
Met -18	-	initiating methionine
Gly -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	279135.3
Non-Polymers*	Number of molecules	8
	Total formula weight	926.7
All*	Total formula weight	280062.0

*Water molecules are not included.