7BYX

Crystal structure of exo-beta-1,3-galactanase from Phanerochaete chrysosporium Pc1,3Gal43A E208A with beta-1,3-galactotriose

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D	Galactan 1,3-beta-galactosidase	polymer	427	45477.6	4	UniProt (Q50KB2) Pfam (PF04616) In PDB	Phanerochaete chrysosporium (White-rot fungus)	exo-beta-1,3-galactanase
2	E, F, G, K, Q	alpha-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		586.5	5	In PDB GlyTouCan (G62182OO)
3	H, L, P, S	beta-D-galactopyranose-(1-3)-beta-D-galactopyranose-(1-3)-beta-D-galactopyranose	branched		504.4	4	In PDB GlyTouCan (G59103JC)
4	I, N	2-acetamido-2-deoxy-alpha-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	2	In PDB GlyTouCan (G07375KG)
5	J, O, R	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	3	In PDB GlyTouCan (G42666HT)
6	M	beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		586.5	1	In PDB GlyTouCan (G15407YE)
7	B, C, D, A	CALCIUM ION	non-polymer		40.1	4	Chemie (CA)
8	B, C, A, D	ACETATE ION	non-polymer		59.0	9	Chemie (ACT)
9	A, B, C, D	GLYCEROL	non-polymer		92.1	19	Chemie (GOL)
10	D	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	1	Chemie (NAG)
11		water	water		18.0	1006	Chemie (HOH)

Sequence modifications

A, B, C, D: 22 - 448 (UniProt: Q50KB2)

PDB	External Database	Details
Ala 208	Glu 208	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	181910.5
Branched	Number of molecules	15
	Total formula weight	7659.0
Non-Polymers*	Number of molecules	33
	Total formula weight	2662.7
All*	Total formula weight	192232.2

*Water molecules are not included.