7BYM
Cryo-EM structure of human KCNQ4 with retigabine
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, C, E, G | Green fluorescent protein,Potassium voltage-gated channel subfamily KQT member 4 | polymer | 979 | 109327.8 | 4 | UniProt (P42212) UniProt (P56696) Pfam (PF01353) Pfam (PF00520) Pfam (PF03520) In PDB | Aequorea victoria (Jellyfish) | KQT-like 4,Potassium channel subunit alpha KvLQT4,Voltage-gated potassium channel subunit Kv7.4 |
| 2 | B, D, F, H | Calmodulin-3 | polymer | 149 | 16852.5 | 4 | UniProt (P0DP25) Pfam (PF13499) In PDB | Homo sapiens (Human) | |
| 3 | A, C, E, G | [(2R)-1-octadecanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phospho ryl]oxy-propan-2-yl] (8Z)-icosa-5,8,11,14-tetraenoate | non-polymer | 1047.1 | 4 | Chemie (PT5) | |||
| 4 | A, C, E, G | ethyl N-[2-azanyl-4-[(4-fluorophenyl)methylamino]phenyl]carbamate | non-polymer | 303.3 | 4 | Chemie (FBX) | |||
| 5 | A | POTASSIUM ION | non-polymer | 39.1 | 3 | Chemie (K) | |||
| 6 | water | water | 18.0 | 4 | Chemie (HOH) |
Sequence modifications
A, C, E, G: -253 - -17 (UniProt: P42212)
A, C, E, G: 1 - 695 (UniProt: P56696)
| PDB | External Database | Details |
|---|---|---|
| Met -283 | - | expression tag |
| His -282 | - | expression tag |
| His -281 | - | expression tag |
| His -280 | - | expression tag |
| His -279 | - | expression tag |
| His -278 | - | expression tag |
| His -277 | - | expression tag |
| His -276 | - | expression tag |
| His -275 | - | expression tag |
| Ala -274 | - | expression tag |
| Ala -273 | - | expression tag |
| Asp -272 | - | expression tag |
| Tyr -271 | - | expression tag |
| Lys -270 | - | expression tag |
| Asp -269 | - | expression tag |
| His -268 | - | expression tag |
| Asp -267 | - | expression tag |
| Ile -266 | - | expression tag |
| Asp -265 | - | expression tag |
| Tyr -264 | - | expression tag |
| Lys -263 | - | expression tag |
| Asp -262 | - | expression tag |
| Asp -261 | - | expression tag |
| Asp -260 | - | expression tag |
| Asp -259 | - | expression tag |
| Lys -258 | - | expression tag |
| Ser -257 | - | expression tag |
| Ala -256 | - | expression tag |
| Met -255 | - | expression tag |
| Val -254 | - | expression tag |
| Leu -191 | Phe 64 | engineered mutation |
| Thr -190 | Ser 65 | engineered mutation |
| Thr -148 | Lys 107 | engineered mutation |
| Lys -49 | Ala 206 | engineered mutation |
| Leu -24 | His 231 | engineered mutation |
| Ser -16 | - | linker |
| Gly -15 | - | linker |
| Leu -14 | - | linker |
| Arg -13 | - | linker |
| Ser -12 | - | linker |
| Gly -11 | - | linker |
| Leu -10 | - | linker |
| Glu -9 | - | linker |
| Val -8 | - | linker |
| Leu -7 | - | linker |
| Phe -6 | - | linker |
| Gln -5 | - | linker |
| Gly -4 | - | linker |
| Pro -3 | - | linker |
| Gly -2 | - | linker |
| Gly -1 | - | linker |
| Arg 0 | - | linker |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 8 |
| Total formula weight | 504721.5 | |
| Non-Polymers* | Number of molecules | 11 |
| Total formula weight | 5519.0 | |
| All* | Total formula weight | 510240.5 |
