7BTK
E.coli beta-galactosidase (E537Q) in complex with fluorescent probe KSA01
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C, D | Beta-galactosidase | polymer | 1025 | 116688.5 | 4 | UniProt (P00722) Pfam (PF02837) Pfam (PF00703) Pfam (PF02836) Pfam (PF16353) Pfam (PF02929) In PDB | Escherichia coli K-12 | Beta-gal,Lactase |
| 2 | A, C, B, D | 4-[[2-[(E)-2-[4-[(2S,3R,4S,5R,6R)-6-(hydroxymethyl)-3,4,5-tris(oxidanyl)oxan-2-yl]oxyphenyl]ethenyl]-3,3-dimethyl-2H-indol-1-yl]methyl]benzoic acid | non-polymer | 560.6 | 4 | Chemie (F6L) | |||
| 3 | A, C, B, D | MAGNESIUM ION | non-polymer | 24.3 | 7 | Chemie (MG) | |||
| 4 | D, A, C, B | SODIUM ION | non-polymer | 23.0 | 7 | Chemie (NA) | |||
| 5 | B, D, A, C | DIMETHYL SULFOXIDE | non-polymer | 78.1 | 38 | Chemie (DMS) | |||
| 6 | B | GLYCEROL | non-polymer | 92.1 | 1 | Chemie (GOL) | |||
| 7 | water | water | 18.0 | 1124 | Chemie (HOH) |
Sequence modifications
A, B, C, D: 0 - 1023 (UniProt: P00722)
| PDB | External Database | Details |
|---|---|---|
| Ser -1 | - | expression tag |
| Gln 537 | Glu 538 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 466754.2 | |
| Non-Polymers* | Number of molecules | 57 |
| Total formula weight | 5634.7 | |
| All* | Total formula weight | 472388.9 |
