7BG4

Multidrug resistance transporter BmrA mutant E504A bound with ATP, Mg, and Rhodamine 6G solved by Cryo-EM

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Lipid A export ATP-binding/permease protein MsbA	polymer	599	65747.1	2	UniProt (A0A164TVX9) Pfam (PF00664) Pfam (PF00005) UniProt (by SIFTS) (O06967) In PDB	Bacillus subtilis
2	A	ADENOSINE-5'-TRIPHOSPHATE	non-polymer		507.2	2	Chemie (ATP)
3	A, B	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
4	A, B	RHODAMINE 6G	non-polymer		443.6	2	Chemie (RHQ)

Sequence modifications

A, B: 1 - 589 (UniProt: A0A164TVX9)

PDB	External Database	Details
Met -9	-	initiating methionine
Ser -8	-	expression tag
Ser -7	-	expression tag
Ser -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag
Ala 504	Glu 504	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	131494.3
Non-Polymers*	Number of molecules	6
	Total formula weight	1950.1
All*	Total formula weight	133444.4

*Water molecules are not included.