6ZJ1

Structure of an inactive E404Q variant of the catalytic domain of human endo-alpha-mannosidase MANEA in complex with tetrasaccharide N-glycan fragment and hexatungstotellurate(VI) TEW

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	AAA	Glycoprotein endo-alpha-1,2-mannosidase	polymer	382	44782.1	1	UniProt (Q5SRI9) Pfam (PF16317) In PDB	Homo sapiens (Human)	hEndo,Mandaselin
2	AdA	alpha-D-glucopyranose-(1-3)-alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-2)-methyl alpha-D-mannopyranoside	branched		680.6	1	In PDB GlyTouCan (G58365BL)
3	AAA	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)
4	AAA	6-tungstotellurate(VI)	non-polymer		1614.6	2	Chemie (TEW)
5		water	water		18.0	133	Chemie (HOH)

Sequence modifications

AAA: 98 - 462 (UniProt: Q5SRI9)

PDB	External Database	Details
Met 81	-	initiating methionine
Asn 82	-	expression tag
His 83	-	expression tag
Lys 84	-	expression tag
Val 85	-	expression tag
His 86	-	expression tag
His 87	-	expression tag
His 88	-	expression tag
His 89	-	expression tag
His 90	-	expression tag
His 91	-	expression tag
Ile 92	-	expression tag
Glu 93	-	expression tag
Gly 94	-	expression tag
Arg 95	-	expression tag
His 96	-	expression tag
Met 97	-	expression tag
Gln 404	Glu 404	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	44782.1
Branched	Number of molecules	1
	Total formula weight	680.6
Non-Polymers*	Number of molecules	3
	Total formula weight	3253.6
All*	Total formula weight	48716.3

*Water molecules are not included.