6YN2
Crystal structure of Renilla reniformis luciferase variant RLuc8-W121F/E144Q in complex with a coelenteramide (the postcatalytic enzyme-product complex)
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A, B | Coelenterazine h 2-monooxygenase | polymer | 317 | 36907.2 | 2 | UniProt (P27652) Pfam (PF00561) In PDB | Renilla reniformis (Sea pansy) | Renilla-luciferin 2-monooxygenase,Renilla-type luciferase |
2 | A, B | N-[3-BENZYL-5-(4-HYDROXYPHENYL)PYRAZIN-2-YL]-2-(4-HYDROXYPHENYL)ACETAMIDE | non-polymer | 411.5 | 2 | Chemie (CEI) | |||
3 | A | ACETATE ION | non-polymer | 59.0 | 1 | Chemie (ACT) | |||
4 | B | GLYCEROL | non-polymer | 92.1 | 1 | Chemie (GOL) | |||
5 | B | POTASSIUM ION | non-polymer | 39.1 | 1 | Chemie (K) | |||
6 | water | water | 18.0 | 507 | Chemie (HOH) |
Sequence modifications
A, B: 1 - 311 (UniProt: P27652)
PDB | External Database | Details |
---|---|---|
Thr 55 | Ala 55 | conflict |
Phe 121 | Trp 121 | engineered mutation |
Ala 124 | Cys 124 | conflict |
Ala 130 | Ser 130 | conflict |
Arg 136 | Lys 136 | conflict |
Met 143 | Ala 143 | conflict |
Gln 144 | Glu 144 | engineered mutation |
Val 185 | Met 185 | conflict |
Leu 253 | Met 253 | conflict |
Leu 287 | Ser 287 | conflict |
His 312 | - | expression tag |
His 313 | - | expression tag |
His 314 | - | expression tag |
His 315 | - | expression tag |
His 316 | - | expression tag |
His 317 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 2 |
Total formula weight | 73814.3 | |
Non-Polymers* | Number of molecules | 5 |
Total formula weight | 1013.1 | |
All* | Total formula weight | 74827.5 |