6WBJ
High resolution crystal structure of mRECK(CC4) in fusion with engineered MBP
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A | Maltodextrin-binding protein,Reversion-inducing cysteine-rich protein with Kazal motifs fusion | polymer | 447 | 49096.5 | 1 | UniProt (A0A376KDN7) UniProt (Q9Z0J1) Pfam (PF01547) UniProt (by SIFTS) (P0AEX9) In PDB | Escherichia coli | mRECK |
2 | B | alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose | branched | 342.3 | 1 | In PDB BIRD (PRD_900001) GlyTouCan (G07411ON) | alpha-maltose | ||
3 | A | SULFATE ION | non-polymer | 96.1 | 1 | Chemie (SO4) | |||
4 | A | ZINC ION | non-polymer | 65.4 | 2 | Chemie (ZN) | |||
5 | A | GLYCEROL | non-polymer | 92.1 | 1 | Chemie (GOL) | |||
6 | water | water | 18.0 | 504 | Chemie (HOH) |
Sequence modifications
A: 5 - 368 (UniProt: A0A376KDN7)
A: 373 - 437 (UniProt: Q9Z0J1)
PDB | External Database | Details |
---|---|---|
Met 1 | - | initiating methionine |
Ala 2 | - | expression tag |
Lys 3 | - | expression tag |
Thr 4 | - | expression tag |
Ala 84 | Asp 108 | engineered mutation |
Ala 85 | Lys 109 | engineered mutation |
Ala 174 | Glu 198 | engineered mutation |
Ala 175 | Asn 199 | engineered mutation |
His 217 | Ala 241 | engineered mutation |
His 221 | Lys 245 | engineered mutation |
Ala 241 | Lys 265 | engineered mutation |
Val 314 | Ala 338 | engineered mutation |
Val 319 | Ile 343 | engineered mutation |
Ala 364 | Lys 388 | engineered mutation |
Ala 365 | Asp 389 | engineered mutation |
Asn 369 | - | linker |
Ala 370 | - | linker |
Ala 371 | - | linker |
Ala 372 | - | linker |
PDB | External Database | Details |
---|---|---|
Gly 438 | - | expression tag |
Thr 439 | - | expression tag |
His 440 | - | expression tag |
His 441 | - | expression tag |
His 442 | - | expression tag |
His 443 | - | expression tag |
His 444 | - | expression tag |
His 445 | - | expression tag |
His 446 | - | expression tag |
His 447 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 1 |
Total formula weight | 49096.5 | |
Branched | Number of molecules | 1 |
Total formula weight | 342.3 | |
Non-Polymers* | Number of molecules | 4 |
Total formula weight | 319.0 | |
All* | Total formula weight | 49757.7 |