6WBH

Crystal structure of mRECK(CC4) in fusion with engineered MBP at medium resolution

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Maltodextrin-binding protein,Reversion-inducing cysteine-rich protein with Kazal motifs fusion	polymer	447	49096.5	1	UniProt (A0A376KDN7) UniProt (Q9Z0J1) Pfam (PF01547) UniProt (by SIFTS) (P0AEX9) In PDB	Escherichia coli	mRECK
2	B	alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose	branched		342.3	1	In PDB BIRD (PRD_900001) GlyTouCan (G07411ON)		alpha-maltose
3	A	CHLORIDE ION	non-polymer		35.5	1	Chemie (CL)
4	A	ZINC ION	non-polymer		65.4	2	Chemie (ZN)
5		water	water		18.0	218	Chemie (HOH)

Sequence modifications

A: 5 - 368 (UniProt: A0A376KDN7)

PDB	External Database	Details
Met 1	-	initiating methionine
Ala 2	-	expression tag
Lys 3	-	expression tag
Thr 4	-	expression tag
Ala 84	Asp 108	engineered mutation
Ala 85	Lys 109	engineered mutation
Ala 174	Glu 198	engineered mutation
Ala 175	Asn 199	engineered mutation
His 217	Ala 241	engineered mutation
His 221	Lys 245	engineered mutation
Ala 241	Lys 265	engineered mutation
Val 314	Ala 338	engineered mutation
Val 319	Ile 343	engineered mutation
Ala 364	Lys 388	engineered mutation
Ala 365	Asp 389	engineered mutation
Asn 369	-	linker
Ala 370	-	linker
Ala 371	-	linker
Ala 372	-	linker

A: 373 - 437 (UniProt: Q9Z0J1)

PDB	External Database	Details
Gly 438	-	expression tag
Thr 439	-	expression tag
His 440	-	expression tag
His 441	-	expression tag
His 442	-	expression tag
His 443	-	expression tag
His 444	-	expression tag
His 445	-	expression tag
His 446	-	expression tag
His 447	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	49096.5
Branched	Number of molecules	1
	Total formula weight	342.3
Non-Polymers*	Number of molecules	3
	Total formula weight	166.3
All*	Total formula weight	49605.0

*Water molecules are not included.