6W5V

NPC1-NPC2 complex structure at pH 5.5

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	NPC intracellular cholesterol transporter 1	polymer	1311	146083.7	1	UniProt (O15118) Pfam (PF16414) Pfam (PF12349) Pfam (PF02460) In PDB	Homo sapiens (Human)	Niemann-Pick C1 protein
2	D	NPC intracellular cholesterol transporter 2	polymer	161	17932.6	1	UniProt (P61916) Pfam (PF02221) In PDB	Homo sapiens (Human)	Epididymal secretory protein E1,Human epididymis-specific protein 1,He1,Niemann-Pick disease type C2 protein
3	B, E, F, G	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	4	In PDB GlyTouCan (G42666HT)
4	C	beta-D-mannopyranose-(1-3)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		586.5	1	In PDB GlyTouCan (G44266EX)
5	A, D	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	10	Chemie (NAG)
6	A, D	CHOLESTEROL	non-polymer		386.7	3	Chemie (CLR)

Sequence modifications

A: 1 - 1278 (UniProt: O15118)

PDB	External Database	Details
Leu 1279	-	expression tag
Glu 1280	-	expression tag
Gly 1281	-	expression tag
Ser 1282	-	expression tag
Asp 1283	-	expression tag
Glu 1284	-	expression tag
Val 1285	-	expression tag
Asp 1286	-	expression tag
Ala 1287	-	expression tag
Gly 1288	-	expression tag
Ser 1289	-	expression tag
His 1290	-	expression tag
His 1291	-	expression tag
His 1292	-	expression tag
His 1293	-	expression tag
His 1294	-	expression tag
His 1295	-	expression tag
His 1296	-	expression tag
His 1297	-	expression tag
His 1298	-	expression tag
His 1299	-	expression tag
Gly 1300	-	expression tag
Ser 1301	-	expression tag
Val 1302	-	expression tag
Glu 1303	-	expression tag
Asp 1304	-	expression tag
Tyr 1305	-	expression tag
Lys 1306	-	expression tag
Asp 1307	-	expression tag
Asp 1308	-	expression tag
Asp 1309	-	expression tag
Asp 1310	-	expression tag
Lys 1311	-	expression tag

D: 1 - 151 (UniProt: P61916)

PDB	External Database	Details
Leu 152	-	expression tag
Glu 153	-	expression tag
His 154	-	expression tag
His 155	-	expression tag
His 156	-	expression tag
His 157	-	expression tag
His 158	-	expression tag
His 159	-	expression tag
His 160	-	expression tag
His 161	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	164016.3
Branched	Number of molecules	5
	Total formula weight	2284.1
Non-Polymers*	Number of molecules	13
	Total formula weight	3372.0
All*	Total formula weight	169672.5

*Water molecules are not included.